Skip to main content
SpringerLink
Log in

Isolation of resveratrol from peanut sprouts, radioiodination and investigation of its bioactivity on neuroblastoma cell lines

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

Recently, natural antioxidant substances have been purified in a significant increasing incline from different plants for diagnosis and treatment options. In the current study, Resveratrol (RES) was isolated and radioiodinated with iodine-131 ([131I]iodo-RES). Cell culture studies were conducted on neuroblastoma cells (SY-SH5Y and SK-N-AS) to investigate the bioavailability of [131I]iodo-RES. The radioiodination yield of RES was 98.81 ± 0.37% (n = 6). Uptake values up to 25% were observed notably on SK-N-AS cells until 24 h. Briefly; the current study will contribute to the development of novel radiolabeled plant origin agents for the imaging of neuroblastoma cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Preedy V, Ronald R, Watson R, Patel V (2011) Nuts & seeds in health and disease prevention. Academic Press, Elsevier Inc, Amsterdam

    Google Scholar 

  2. Guimón J, Guimón P (2012) How ready-to-use therapeutic food shapes a new technological regime to treat child malnutrition. Technol Forecast Soc Change. 79(7):1319–1327

    Article  Google Scholar 

  3. Arya SS, Salve AR, Chauhan S (2016) Peanuts as functional food: a review. J Food Sci Technol 53(1):31–41

    Article  CAS  Google Scholar 

  4. Limmongkon A, Nopprang P, Chaikeandee P, Somboon T, Wongshaya P, Pilaisangsuree V (2018) LC-MS/MS profiles and interrelationships between the anti-inflammatory activity, total phenolic content and antioxidant potential of Kalasin 2 cultivar peanut sprout crude extract. Food Chem 239:569–578

    Article  CAS  Google Scholar 

  5. Xiong Q, Zhang Q, Zhang D, Shi Y, Jiang C, Shi X (2014) Preliminary separation and purification of resveratrol from extract of peanut (Arachis hypogaea) sprouts by macroporous adsorption resins. Food Chem 145:1–7

    Article  CAS  Google Scholar 

  6. Gaballah H, Zakaria S, Elbatsh M, Tahoon NM (2016) Modulatory effects of resveratrol on endoplasmic reticulum stress-associated apoptosis and oxido-inflammatory markers in a rat model of rotenone-induced Parkinson’s disease. Chem Biol Interact 251:10–16

    Article  CAS  Google Scholar 

  7. Van Ginkel PR, Sareen D, Subramanian L, Walker Q, Darjatmoko SR, Lindstorm MJ, Kulkarni A, Albert DM, Polans AS (2007) Resveratrol inhibits tumor growth of human neuroblastoma and mediates apoptosis by directly targeting mitochondria. Clin Cancer Res 13(17):5162–5169

    Article  Google Scholar 

  8. Gambini J, Ingles M, Olaso G, Lopez Grueso R, Bonet Costa V, Gimeno Mallench L, Mas Bargues C, Abdelaziz KM, Gomez Cabrera MC, Vina J, Borras C (2015) Properties of Resveratrol: in vitro and in vivo studies about metabolism, bioavailability, and biological effects in animal models and humans. Oxid Med Cell Longev 837042:1–12

    Article  Google Scholar 

  9. Lertkaeo P, Limmongkon A, Srikummool M, Boonsong T, Supanpaiboon W, Surangkul D (2017) Antioxidative and neuroprotective activities of peanut sprout extracts against oxidative stress in SK-N-SH cells. Asian Pac J Trop Biomed. 7(1):64–69

    Article  Google Scholar 

  10. Wang G, Lei Z, Zhong Q, Wu W, Zhang H, Min T, Wu H, Lai F (2017) Enrichment of caffeic acid in peanut sprouts and evaluation of its in vitro effectiveness against oxidative stress-induced erythrocyte hemolysis. Food Chem 217:332–341

    Article  CAS  Google Scholar 

  11. Jin F, Wu Q, Lu YF, Gong QH, Shi JS (2008) Neuroprotective effect of resveratrol on 6-OHDA-induced Parkinson’s disease in rats. Eur J Pharmacol 600(1–3):78–82

    Article  CAS  Google Scholar 

  12. Peñalver P, Belmonte Reche E, Adan N, Caro M, Mateos Martin ML, Delgado M, Gonzalez Rey E, Morales JC (2018) Alkylated resveratrol prodrugs and metabolites as potential therapeutics for neurodegenerative diseases. Eur J Med Chem 146:123–138

    Article  Google Scholar 

  13. Biber Muftuler FZ, Yurt Kilcar A, Unak P (2015) A perspective on plant origin radiolabeled compounds, their biological affinities and interaction between plant extracts with radiopharmaceuticals. J Radioanal Nucl Chem 306(1):1–9

    Article  CAS  Google Scholar 

  14. Yu M, Liu H, Shi A, Liu L, Wang Q (2016) Preparation of resveratrol-enriched and poor allergic protein peanut sprout from ultrasound treated peanut seeds. Ultrason Sonochem 28:334–340

    Article  CAS  Google Scholar 

  15. Youn CK, Jo ER, Sim JH, Cho S (2017) Peanut sprout extract attenuates cisplatin-induced ototoxicity by induction of the Akt/Nrf2-mediated redox pathway. Int J Pediatr Otorhinolaryngol 92:61–66

    Article  Google Scholar 

  16. Biber Muftuler FZ, Unak P (2017) A perspective on 99mTc and 125/131I labeled receptor targeted compounds and their in vitro/in vivo affinities. J Radioanal Nucl Chem 314(1):1–6

    Article  CAS  Google Scholar 

  17. Dervis E, Yurt Kilcar A, Medine EI, Tekin V, Cetkin B, Uygur E, Biber Muftuler FZ (2017) In Vitro incorporation of radioiodinated Eugenol on adenocarcinoma cell lines (Caco2, MCF7, and PC3). Cancer Biother Radiopharm. 32(3):75–81

    Article  CAS  Google Scholar 

  18. Ozkan BM, Biber Muftuler FZ, Yurt Kilcar A, Medine EI, Unak P (2013) Isolation of hydroxytyrosol from olive leaves extract, radioiodination and investigation of bioaffinity using in vivo/in vitro methods. Radiochim Acta 101:585–593

    CAS  Google Scholar 

  19. Asik FF, Yildiz R, Arioglu HH (2018) The Determination of new peanut varieties for Osmaniye region and their important agronomic and quality characteristics (Koşullarına uygun yeni yerfıstığı çeşitleri ile bunların önemli tarımsal ve kalite özelliklerinin belirlenmesi). KSU J Agric Nat 21(6):825–836

    Google Scholar 

  20. Wang CZ, Qi LW, Yuan CS (2015) Cancer chemoprevention effects of ginger and its active constituents’ potential for new drug discovery. Am J Chin Med 43(7):1351–1363

    Article  CAS  Google Scholar 

  21. Unak T, Akgün Z, Yildirim Y, Duman Y, Erenel G (2001) Self radioiodination of iodogen. Appl Radiat Isot 54:749–752

    Article  CAS  Google Scholar 

  22. Oh WY, Shahidi F (2017) Lipophilization of resveratrol and effects on antioxidant activities. J Agric Food Chem 65(39):8617–8625

    Article  CAS  Google Scholar 

  23. Ohtsuki S, Terasaki T (2007) Contribution of carrier-mediated transport systems to the blood–brain barrier as a supporting and protecting interface for the brain; importance for CNS drug discovery and development. Pharm Res 24(9):1745–1758

    Article  CAS  Google Scholar 

  24. Abla MJ, Banga AK (2013) Quantification of skin penetration of antioxidants of varying lipophilicity. Int J Cosmet Sci 35(1):19–26

    Article  CAS  Google Scholar 

  25. Murtaza G, Latif U, Najam-ul-Haq M, Sajjad A, Karım S, Akhtar M, Hussain I (2013) Resveratrol: an active natural compound in red wines for health. J Food Drug Anal. 21(1):1–12

    CAS  Google Scholar 

  26. Limmongkon A, Janhom P, Ampika A, Kawpanuk M, Nopprang P, Poohadsuan J, Samboon T, Saijeen S, Surangkul Srikummool M, Boonsong T (2017) Antioxidant activity, total phenolic, and resveratrol content in five cultivars of peanut sprouts. Asian Pac J Trop Biomed 7(4):332–338

    Article  Google Scholar 

  27. Bowers JL, Tyulmenkov VV, Jernigan SC, Klinge CM (2000) Resveratrol acts as a mixed agonist/antagonist for estrogen receptors α and β. Endocrinology 141(10):3657–3667

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Present study was supported by Ege University Scientific Research Projects, Ege University/TURKEY (Project Number: FYL-2018-20081). Authors thank to Mr. Resat YILDIZ for sending the seeds from “Osmaniye Oil Seeds Research Institute”, Osmaniye/TURKEY. Also, authors thank to Dr. Volkan TEKIN and Msc. Cansu KAYAS for their technical help and thank to Mr. Ali Rıza GULMEZ for support in growing peanut sprouts.

Author information

Authors and Affiliations

  1. Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100, Bornova, Izmir, Turkey

    Kadriye Busra Karatay, Ayfer Yurt Kilcar, Ozge Kozgus Guldu, Emin Ilker Medine & Fazilet Zumrut Biber Muftuler

Authors
  1. Kadriye Busra Karatay
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ayfer Yurt Kilcar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ozge Kozgus Guldu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Emin Ilker Medine
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fazilet Zumrut Biber Muftuler
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fazilet Zumrut Biber Muftuler.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Karatay, K.B., Yurt Kilcar, A., Kozgus Guldu, O. et al. Isolation of resveratrol from peanut sprouts, radioiodination and investigation of its bioactivity on neuroblastoma cell lines. J Radioanal Nucl Chem 325, 75–84 (2020). https://doi.org/10.1007/s10967-020-07222-5

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-020-07222-5

Keywords

Search

Navigation