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Dynamic modulations of the MDA-MB-231 secretions at low dose radiation

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Abstract

The goal was to investigate the time dynamic nature of the secreted protein profiles at 15–240 kDa, expressed by MDA-MB-231 breast cancer cells, exposed to 240 ± 12 mGy dose. After 2 h, a variety of proteins in the supernatant reduced their concentration up to 10–12 h, as an early event. After 24 h, up- and down-regulations of the secretions of several factors were postulated, indicating significant radio-induced changes. At a late event, in 48–96 h, the findings pointed out to distinct radio-induced modulations characterizing early and late profiles. Such findings suggest dynamic secretome behavior, possibly related to distinct MDA-MB-231 phenotypes.

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Acknowledgements

The authors are thankful to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), due to the financial aid from process 456719/2013–0 REBRAT-SUS, due to scholarships and DTI scholar of Iassudara Garcia, and the Coordenação de Apoio aos Programas de Ensino Superior (CAPES). The authors acknowledge the Physiology and Immunology Department to have allowed the use of the Multiskan FC Thermo Scientific reader.

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  1. Nuclear Engineering Department, Universidade Federal de Minas Gerais, Antonio Carlos Avenue, 6627, Building 4, Belo Horizonte, MG, 31270901, Brazil

    T. P. R. Campos, I. G. Almeida & T. M. Mendes

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Campos, T.P.R., Almeida, I.G. & Mendes, T.M. Dynamic modulations of the MDA-MB-231 secretions at low dose radiation. J Radioanal Nucl Chem 324, 929–940 (2020). https://doi.org/10.1007/s10967-020-07139-z

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