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99mTc-Labeled human and camel lactoferrin for detection of Staphylococcus aureus infections

  • Alifa Kanoun
  • Issam B. Salem
  • Frida Darghouth
  • Amine Asmi
  • Halima El Hatmi
  • Isabelle ADT
  • Mouldi Saidi
Article
  • 39 Downloads

Abstract

Human and camel lactoferrin, were labeled with technetium-99m using a simple technique that yielded a labelling efficiency of 98% and stable complexes over time. The camel Lactoferrin was purified. The proteins were investigated for targeting infections in experimental animals. The biodistribution of those labeled proteins in mice infected with Staphylococcus aureus at the femoral muscle was investigated and test ratios of 3.43 and 3.71 were obtained after 60 min for the camel and the human Lactoferrin, respectively. These values were compared to each other and to those reported in the literature, leading us to conclude that the radiotracers are efficient.

Keywords

99mTechnetium Staphylococcus aureus Human lactoferrin Camel lactoferrin Infectious sites 

Notes

Acknowledgements

The authors would like to thank the staff of Pasteur Institute of Tunisia for their cooperation and assistance with some. The authors are also grateful to Pr BEN SLIMANE of the Salah Azaiez Institute of Tunis for generously providing us with radioactive technetium.

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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2018

Authors and Affiliations

  • Alifa Kanoun
    • 1
    • 2
    • 3
  • Issam B. Salem
    • 1
  • Frida Darghouth
    • 2
  • Amine Asmi
    • 1
  • Halima El Hatmi
    • 4
  • Isabelle ADT
    • 5
  • Mouldi Saidi
    • 1
  1. 1.Research Unit, Application of Nuclear Techniques in the Fields of Health, Agriculture and EnvironmentNational Centre for Nuclear Science and Technology (CNSTN)ArianaTunisia
  2. 2.Corporation of Pharmaceutical Industry Ibn Al BaytarLa SoukraTunisia
  3. 3.Faculty of Sciences of BizerteZarzounaTunisia
  4. 4.Laboratoire Elevage et faune SauvageInstitut des Régions AridesMedenineTunisia
  5. 5.ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires)Univ Lyon, Université Claude Bernard Lyon 1Bourg en BresseFrance

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