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In vitro analysis of radioprotective effect of monoterpenes


Monoterpenes are naturally occurring hydrocarbons composed of two units of isoprenes. They exhibit antioxidant activity to scavenge reactive oxygen species, such as hydroxyl radicals. We investigated the potential of monoterpenes such as thymol, linalool, and menthol to act as radioprotectants. The proliferation of EL4 cells, a mouse lymphoma cell line, treated with linalool at a concentration of 500 μM or more was not affected by X-ray irradiation. Plasmid-nicking assay performed using formamidopyrimidine-DNA glycosylase showed that linalool prevented single strand breaks and oxidized purines on pUC19 plasmid DNA. These findings indicate that linalool has the ability to scavenge reactive oxygen species and is a potential radioprotector.

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We thank the staff of Department of Animal Resources, Advanced Science Research Center, Okayama University for use of X-ray generator MBR-1520R. We also thank Ms. T. Terada for preparation of the manuscript. This work was supported in part by the Grant for Strategies for the Efficient Operation of Okayama University.

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Correspondence to Toshiro Ono.

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Kudo, Ki., Hanafusa, T. & Ono, T. In vitro analysis of radioprotective effect of monoterpenes. J Radioanal Nucl Chem 313, 169–174 (2017).

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  • Monoterpenes
  • Linalool
  • X-ray irradiation
  • Reactive oxygen species
  • SSB