In vitro analysis of radioprotective effect of monoterpenes
- 377 Downloads
Monoterpenes are naturally occurring hydrocarbons composed of two units of isoprenes. They exhibit antioxidant activity to scavenge reactive oxygen species, such as hydroxyl radicals. We investigated the potential of monoterpenes such as thymol, linalool, and menthol to act as radioprotectants. The proliferation of EL4 cells, a mouse lymphoma cell line, treated with linalool at a concentration of 500 μM or more was not affected by X-ray irradiation. Plasmid-nicking assay performed using formamidopyrimidine-DNA glycosylase showed that linalool prevented single strand breaks and oxidized purines on pUC19 plasmid DNA. These findings indicate that linalool has the ability to scavenge reactive oxygen species and is a potential radioprotector.
KeywordsMonoterpenes Linalool X-ray irradiation Reactive oxygen species SSB
We thank the staff of Department of Animal Resources, Advanced Science Research Center, Okayama University for use of X-ray generator MBR-1520R. We also thank Ms. T. Terada for preparation of the manuscript. This work was supported in part by the Grant for Strategies for the Efficient Operation of Okayama University.
- 4.Hofer M, Falk M, Komůrková D, Falková I, Bačíková A, Klejdus B, Pagáčová E, Štefančíková L, Weiterová L, Angelis KJ, Kozubek S, Dušek L, Galbavý S (2016) Two new faces of amifostine: protector from DNA damage in normal cells and inhibitor of DNA repair in cancer cells. J Med Chem 59:3003–3017CrossRefGoogle Scholar
- 14.OECD Screening Information DataSet (SIDS) (2002) High production volume chemicals (processed by UNEP chemicals), SIDS Initial Assessment Report For SIAM 14Google Scholar
- 15.OECD Screening Information DataSet (SIDS) (2003) High production volume chemicals (processed by UNEP chemicals), SIDS Initial Assessment Report For SIAM 16Google Scholar
- 17.Tchou J, Bodepudi V, Shibutani S, Antoshechkin I, Miller J, Grollman AP, Johnson F (1994) Substrate specificity of Fpg protein. J Biol Chem 269:15318–15324Google Scholar
- 22.Iwasaki K, Zheng YW, Murata S, Ito H, Nakayama K, Kurokawa T, Sano N, Nowatari T, Villareal MO, Nagano YN, Isoda H, Matsui H, Ohkohchi N (2016) Anticancer effect of linalool via cancer-specific hydroxyl radical generation in human colon cancer. World J Gastroent 22(44):9765–9774CrossRefGoogle Scholar