Skip to main content
SpringerLink
Log in

Comparative biological parameters for the evaluation of common renal pharmaceutical preparations: 99mTc-DTPA, 99mTc-GH, and 99mTc-DMSA

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

This work is concerned on establishing the necessary comparative quality control biological parameters for the evaluation of locally produced renal pharmaceutical preparations viz. tin complexes of 99mTc-DTPA, 99mTc-GH, and 99mTc-DMSA using gel chromatography column scanning techniques. Radiochemical purity, labeling yields, organ distribution, blood clearance and plasma protein binding were used as a selected indicator for quality control. The higher renal accumulation and tissue distribution were apparent with 99mTc-DTPA in mice, while blood retention was considerably low. The blood clearance of 99mTc-DMSA was relatively slow, while that of 99mTc-GH was rapid. The binding of 99mTc-DTPA, 99mTc-GH and 99mTc-DMSA with plasma protein were found to be 5, 65, and 95 % at 1.0 h, respectively. The stability of these cold kits was not affected on storing at 37 °C for 30 days.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Atkins HL, Cardinale KG, Eckelman WC, Hauser W, Klopper JF, Richards P (1971) Evaluation of 99mTc-DTPA by three different method. Radiology 98:674–677

    Article  CAS  Google Scholar 

  2. Hauser W, Atkins HL, Nelson KG, Richards P (1970) 99mTc-DTPA: a new radiopharmaceutical for brain and kidney scanning. Radiology 94:679–684

    Article  CAS  Google Scholar 

  3. Jan SU, Abbass HG (2013) Preparation and evaluation of 99mTc-DMSA lyophilized kit for renal imaging. Pak J Pharm Sci 26(3):547–551

    CAS  Google Scholar 

  4. Dostbil Z, Pembegül N, Küçüköner M, Bozkurt Y, Sancaktutar AA, Yildiz S, Tekba G (2011) Comparison of split renal function measured by 99mTc-DTPA, 99mTc-MAG3 and 99mTc-DMSA renal scintigraphies in paediatric age groups. Clin Rev Opin 3(2):20–25

    CAS  Google Scholar 

  5. 99mTc radiopharmaceuticals: manufacture of kits (2008) Technical reports series, ISSN 0074–1914; no. 466, International Atomic Energy Agency, Vienna

  6. Antoniou KM, Malagari K, Tzanakis N, Perisinakis K, Symvoulakis EK, Karkavitsas N, Siafakas NM, Bouros D (2006) Clearance of 99mTc-DTPA and HRCT findings in the evaluation of patients with idiopathic pulmonary fibrosis. BMC Pulm Med 6:4

    Article  Google Scholar 

  7. Russell CD, Rowell K, Scott JW (1986) Quality control of 99mTc DTPA: correlation of analytic tests with in vivo protein binding in man. J Nucl Med 27:560–562

    CAS  Google Scholar 

  8. Vorne M, Sakki S, Järvi K, Vähätalo S (1982) 99mTc glucoheptonate in detection of lung tumors. J Nucl Med 23(3):250–254

    CAS  Google Scholar 

  9. Guerra U, Pizzocaro C, Terzi A, Giubbini R, Bestagno M (1988) The use of 99mTc(V)DMSA as imaging for the medullary thyroid carcinoma (MTC). J Nucl Med Allied Sci 32(4):242–247

    CAS  Google Scholar 

  10. Russell CD, Bischoff PG, Rowell KL (1983) Quality control of 99mTc-DTPA for measurement of glomerular filtration. J Nucl Med 24:722

    CAS  Google Scholar 

  11. Mckusick KA, Malmud LS, Kirshner PT (1973) An interesting artifact in radionuclide imaging of the kidneys. J Nucl Med 14:113

    CAS  Google Scholar 

  12. Hosain FJ (1974) Quality control of 99mTc-DTPA by double-tracer clearance technique. Nucl Med 15:442

    CAS  Google Scholar 

  13. Rehling M (1988) Stability, protein binding and clearance studies of [99mTc]DTPA. Evaluation of a commercially available dry-kit. Scand J Clin Lab Invest 48(7):603–609

    Article  CAS  Google Scholar 

  14. Konikowski T, Glenn HJ, Haynie TP (1972) Renal clearance and brain tumor localization in mice of 99mTc compounds of (Sn), DTPA, (iron-ascorbic acid) DTPA, and iron-ascorbic acid. J Nucl Med 13:834

    CAS  Google Scholar 

  15. Barthez PY, Hornof WJ, Cowgill LD, Neal LA, Mickel P (1998) Comparison between the scintigraphic uptake and plasma clearance of 99mTc-diethylenetriaminepentacetic acid (DTPA) for the evaluation of the glomerular filtration rate in dogs. Vet Radiol Ultrasound 39:470–474

    Article  CAS  Google Scholar 

  16. Rehling M, Nielsen LE, Marqversen J (2001) Protein binding of 99mTc DTPA compared with other GFR tracers. Nucl Med Commun 22:617–623

    Article  CAS  Google Scholar 

  17. Ikeda I, Inoue O, Kurata K (1977) Preparation of various 99mTc dimercaptosuccinate complex and their evaluation as radiotracers. J Nucl Med 18:1222–1229

    CAS  Google Scholar 

  18. Mohsan F, Mansoor MS, Ahmed M, Khwar A, Ahmed S (2008) Comparison of stabilizers, Myo-inositol and D-mannitol for 99mTc-DMSA (III). J Chem Soc Pak 30(6):907

    Google Scholar 

  19. Gorla AKR, Agrawal K, Sood A, Bhattacharya A, Mittal BR (2014) Differential uptake of 99mTc DMSA and 99mTc EC in renal tubular disorders: report of two cases and review of the literature. Indian J Nucl Med 29(3):160–162

    Article  Google Scholar 

  20. Yee CA, Lee HB, Blaufox MD (1981) 99mTc DMSA renal uptake: influence of biochemical and physiologic factors. J Nucl Med 22:1054

    CAS  Google Scholar 

  21. Boyd RE, Robson J, Hunt FC (1973) 99mTc-gluconate complex for renal Scientography. British J Radiol 24:604

    Article  Google Scholar 

  22. Fritzberg AR, Klingensmith WC, Whitney WP, Kuni CC (1981) Chemical and biological studies of 99mTc N, N′-Bis(mercaptoacetamido)-ethylenediamine: a potential replacement for 131I iodohippurate. J Nucl Med 22:258–263

    CAS  Google Scholar 

  23. Atkins HL, Cardinale KG, Eckelman WC, Hauser W, Klopper JF, Richards P (1971) Evaluation of 99mTc-DTPA prepared by three different methods. Radiology 98:674–677

    Article  CAS  Google Scholar 

  24. Klopper JF, Hauser W, Atkins HL (1972) Evaluation of 99mTc DTPA for the measurement of GFR. J Nucl Med 13:107

    CAS  Google Scholar 

  25. McAfee JG, Gagne G, Atkins HL, Kirchner PT, Reba RC, Blaufox MD, Smith EM (1979) Biological distribution and excretion of DTPA labeled with 99mTc and 111In. J Nucl Med 20:1273

    CAS  Google Scholar 

  26. Kempi V, Persson RBR (1975) Dry-kit preparation quality control and clearance studies. Nucl Med (Stuttg.) 13:389

    CAS  Google Scholar 

  27. Carlsen JE, Moller ML, Lund JO (1980) Comparison of four commercial 99mTc-DTPA preparation for the measurement of GFR: concise communication. J Nucl Med 21:126

    CAS  Google Scholar 

  28. Arnold RW, Subramanian G, McAfee JG (1975) Comparison of 99mTc complexes for renal imaging. J Nucl Med 16:357–367

    CAS  Google Scholar 

  29. Vanlic-Razumenic N, Malesevic M, Stefanovic LJ (1979) Comparative chemical, biological and clinical studies of 99mTc-glucoheptonate and 99mTc-dimercaptosuccinate as used in renal scintigraphy. Nucl Med (Stuttg) 18:40

    CAS  Google Scholar 

  30. Hagan PL, Chauncey DM, Halpern SE, Ayres PR (1977) 99mTc-thiomalic acid complex a non stannous chelate for renal scanning. J Nucl Med 18:353

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Directorate of Research and Nuclear Applications, Ministry of Science Technology, Al-Jadriyia, P.O. 765, Baghdad, Iraq

    Hisham M. A. Al-Azzawi & Jameel M. Badi

  2. Environmental Research Centre, The University of Technology, Baghdad, Iraq

    Saadi M. D. Al-Nuzal

Authors
  1. Hisham M. A. Al-Azzawi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Saadi M. D. Al-Nuzal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jameel M. Badi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Saadi M. D. Al-Nuzal.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Al-Azzawi, H.M.A., Al-Nuzal, S.M.D. & Badi, J.M. Comparative biological parameters for the evaluation of common renal pharmaceutical preparations: 99mTc-DTPA, 99mTc-GH, and 99mTc-DMSA. J Radioanal Nucl Chem 307, 123–130 (2016). https://doi.org/10.1007/s10967-015-4173-7

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-015-4173-7

Keywords

Search

Navigation