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The novel preparation of 99mTc(I)-labeled human serum albumin (HSA) nanoparticles as a SPECT imaging agent

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Abstract

The purpose of this study was to develop a convenient and robust method for preparation of 99mTc(I)-labeled human serum albumin nanoparticles (HSA-NPs) as a SPECT agent for tumor imaging. Efficient radiolabeling 99mTc to HSA-NPs was achieved by simply reacting [99mTc(OH2)3(CO)3]+ with the reconstituted HSA-NPs solution. The size and morphology of 99mTc(I)-HSA-NPs were analyzed and found not to significantly change after the preformed HSA-NPs were lyophilized or labeled with 99mTc(I). By incubating 99mTc(I)-HSA-NPs in rat plasma at 37 °C for 30 h, 75 % of the radioactivity were found to be remained in the particles, suggesting good in vitro stability.

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Acknowledgments

This work was supported by Institute of Nuclear Energy Research in Taiwan, National Tsing Hua University, and Ministry of Science and Technology, Taiwan (102-2113-M-007-006-MY2). The authors are grateful to Mrs. Shang-Fang Chang, Instrumentation Center, National Tsing Hua University, Hsinchu, Taiwan, for excellent technical assistance with TEM. The authors wish to acknowledge members in the radiopharmacology laboratory, Division of Isotope Application, Institute of Nuclear Energy Research, Longtan, Taiwan, for the expert technical assistance and helpful discussion. The authors also would like to thank Prof. Bor-Tsung Hsieh, Department of Medical imaging and Radiological Sciences, Central Taiwan University of Science and Technology, Taichung, Taiwan, for supplying a freeze-dryer, and Dr. Kai-Yuan Tzen, Department of Nuclear Medicine, National Taiwan University Hospital, Taipei, Taiwan, for generously providing 99Mo-99mTc generators in this study.

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    Authors and Affiliations

    1. Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan

      Wan-Jou Chen, Feng-Yun J. Huang, Chien-Wen Chang & Jem-Mau Lo

    2. Institute of Nuclear Engineering and Science, National Tsing Hua University, Hsinchu, 30013, Taiwan

      Hsiu-Yu Chang & Jem-Mau Lo

    3. Institute of Nuclear Energy Research, Longtan, 32546, Taiwan

      Te-Wei Lee

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    1. Wan-Jou Chen
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    2. Feng-Yun J. Huang
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    Correspondence to Chien-Wen Chang or Jem-Mau Lo.

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    Wan-Jou Chen and Feng-Yun J. Huang have contributed equally to this work.

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    Chen, WJ., Huang, FY.J., Chang, HY. et al. The novel preparation of 99mTc(I)-labeled human serum albumin (HSA) nanoparticles as a SPECT imaging agent. J Radioanal Nucl Chem 307, 141–150 (2016). https://doi.org/10.1007/s10967-015-4094-5

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    • DOI: https://doi.org/10.1007/s10967-015-4094-5

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