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Standardisation of radioimmunoassay for human insulin employing magnetizable cellulose particles

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Abstract

We describe a convenient and flexible solid phase radioimmunoassay for human insulin employing magnetizable cellulose particles. Anti-porcine insulin antibody was covalently linked to magnetizable cellulose particles to form a stable and economical solid phase immunosorbent system. The tracer was prepared by radioiodinating insulin with 125I using Chloramine-T oxidation method. The analytical sensitivity of assay observed was 5.5 μIU/mL. Intra-assay and inter-assay variations were found to be <12 % along with analytical recovery of 93–109 %. The developed assay can be used for the routine analysis of clinical samples. In addition, concentration of the solid phase magnetizable immunosorbent can be easily varied as per the specific requirement for research purposes.

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Acknowledgments

We thank Dr. A. K. Kohli, Chief Executive, BRIT and Shri. S. S. Sachdev, Senior General Manager, RPhP for their keen interest and support throughout course of this work.

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Authors and Affiliations

  1. Centre for Application of Radioisotopes and Radiation Technology (CARRT), Mangalore University, Mangalagangothri, 574199, India

    R. R. Rasmi, K. Bhasker Shenoy & H. M. Somashekarappa

  2. Radiopharmaceuticals Programme, Board of Radiation and Isotope Technology (BRIT), Vashi Complex, Sector 20, Vashi, Navi Mumbai, 400705, India

    Jayula Sarnaik, V. B. Kadwad & N. Sivaprasad

Authors
  1. R. R. Rasmi
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  2. K. Bhasker Shenoy
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  3. Jayula Sarnaik
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  4. V. B. Kadwad
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  5. H. M. Somashekarappa
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  6. N. Sivaprasad
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Correspondence to K. Bhasker Shenoy.

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Rasmi, R.R., Bhasker Shenoy, K., Sarnaik, J. et al. Standardisation of radioimmunoassay for human insulin employing magnetizable cellulose particles. J Radioanal Nucl Chem 302, 1271–1275 (2014). https://doi.org/10.1007/s10967-014-3539-6

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  • DOI: https://doi.org/10.1007/s10967-014-3539-6

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