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Assessment of the associated particle prompt gamma neutron activation technique for total body nitrogen measurement in vivo

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Abstract

Total body nitrogen (TBN) can be used to estimate total body protein, an important body composition component at the molecular level. A system using the associated particle technique in conjunction with prompt gamma neutron activation analysis has been developed for the measurement of TBN in vivo. The system uses a compact D, T neutron generator (~107 n/s) coupled to an internal alpha-particle detector, and a counting system with six bismuth germinate detectors. 14 Subjects were scanned from shoulders to hips (20 min scan time, <0.4 mSv dose) generating complex spectra dominated by signals from C, O, H, and N, with significant peak overlap. Fractional contributions from these elements to regions of interests (ROI) spanning a 4–8 MeV range were determined by algorithms comparing ratios of interrelated ROIs. In addition, multi-component least squares fitting was done to further resolve individual peak activities (MATLAB R2011b). Total body potassium (TBK) was also measured using a whole body gamma counter. Predicted TBN values, based on fat-free mass estimated from TBK, were compared to measured TBN results. Measured versus predicted results for all subjects were not statistically different. Separating subjects by gender also showed no difference between measured and predicted values. The associated particle system showed good agreement with predicted TBN values, but measurement precision was not better than that commonly seen in traditional prompt gamma thermal neutron activation analysis systems.

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References

  1. Nagai T, Fujii I, Muto H, Inouye T (1969) J Nucl Med 10:192–196

    CAS  Google Scholar 

  2. Wang ZM, Pierson RN Jr, Heymsfield SB (1992) Am J Clin Nutr 56:19–28

    CAS  Google Scholar 

  3. Hedley AA, Ogden CL, Johnson CL, Carroll MD, Curtin LR, Flegal KM (2004) JAMA 291:2847–2850

    Article  CAS  Google Scholar 

  4. Chettle DR, Fremlin JH (1984) Phys Med Biol 29:1011–1043

    Article  CAS  Google Scholar 

  5. Beddoe AH, Zuidmeer A, Hill GL (1984) Phys Med Biol 29:371–383

    Article  CAS  Google Scholar 

  6. Dilmanian FA, Lidofsky LJ, Stamatelatos I, Kamen Y, Yasumura S, Vartsky D, Pierson RN, Weber DA, Moore RI, Ma R (1998) Phys Med Biol 43:339–349

    Article  CAS  Google Scholar 

  7. Hollas CL, Ussery LE, Butterfield KB, Morgado RE (1990) Basic Life Sci 55:395–400

    CAS  Google Scholar 

  8. Gierlik M, Batsch T, Moszynski M, Szczesniak T, Wolski D, Klamra W, Perot B, Perret G (2006) IEEE Trans Nucl Sci 53:1737–1743

    Article  CAS  Google Scholar 

  9. Gordon CM, Peters CW (1990) Int J Radiat Appl Instrum A 41:1111–1116

    Article  CAS  Google Scholar 

  10. Mitra S, Wolff JE, Garrett R, Peters CW (1995) Phys Med Biol 40:1045–1055

    Article  CAS  Google Scholar 

  11. Shypailo RJ, Ellis KJ (2005) J Radioanal Nucl Chem 263:759–765

    Article  CAS  Google Scholar 

  12. Shypailo RJ, Ellis KJ (2008) J Radioanal Nucl Chem 276:71–77

    Article  CAS  Google Scholar 

  13. Ellis KJ, Shypailo RJ (2008) J Radioanal Nucl Chem 276:79–83

    Article  CAS  Google Scholar 

  14. Chichester DL, Simpson JD, Lemchak M (2007) J Radioanal Nucl Chem 271:629–637

    Article  CAS  Google Scholar 

  15. Lim CS, Sowerby BD (2005) J Radioanal Nucl Chem 264:15–19

    Article  CAS  Google Scholar 

  16. Shypailo RJ, Ellis KJ (2011) Phys Med Biol 56:2979–2997

    Article  CAS  Google Scholar 

  17. Baur LA, Allen JR, Humphries IR, Gaskin KJ (2001) In: Jurimae T, Hills AP (eds) Body composition assessment in children and adolescents. Karger, Basel

    Google Scholar 

  18. Perot B, Carasco C, Bernard S et al (2008) Appl Rad Isotopes 66:421–434

    Article  CAS  Google Scholar 

  19. ICRP (1975) Report of the task group on reference man. International commission of radiation protection publication 23. Pergamon, Oxford

    Google Scholar 

Download references

Acknowledgments

This work is a publication of the USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, and Texas Children’s Hospital, Houston, TX. Funding has been provided from the USDA/ARS under Cooperative Agreement No. 58-6250-6-001. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government.

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Authors and Affiliations

  1. USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, 1100 Bates Street, Houston, TX, 77030, USA

    R. J. Shypailo & K. J. Ellis

  2. Division of Nephrology, Baylor College of Medicine, Houston, TX, 77030, USA

    B. Workeneh

Authors
  1. R. J. Shypailo
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  2. B. Workeneh
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  3. K. J. Ellis
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Correspondence to R. J. Shypailo.

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Shypailo, R.J., Workeneh, B. & Ellis, K.J. Assessment of the associated particle prompt gamma neutron activation technique for total body nitrogen measurement in vivo. J Radioanal Nucl Chem 296, 143–148 (2013). https://doi.org/10.1007/s10967-012-2138-7

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  • DOI: https://doi.org/10.1007/s10967-012-2138-7

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