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99mTc-labeling and molecular modeling of short dipeptide glycyl-l-proline

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Abstract

Glycyl-l-proline (Gly-l-Pro) is the main degradation product of collagen and is a good diagnostic tool in various pathological conditions. The aim of this work was to prepare dipeptide Gly-l-Pro labeled with 99mTc. Complex preparation was carried out under alkaline reaction conditions and its stability was assessed 10 and 120 min after preparation. The formation of two types of complex compounds was observed. High-performance liquid chromatography, paper electrophoresis, paper chromatography and thin layer chromatography were employed to monitor the formation of different complexes. Molecular modeling (semi-empirical method) was used to design their structure and composition. First complex cI with formula [TcO(Gly-l-Pro)]−1 is unstable. After 120 min cI is completely transformed to complex cII with formula Tc(Gly-l-Pro)3.

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Acknowledgment

This work was funded by grand from the Ministry of Education of the Slovak Republic number 1/0003/08.

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32, Bratislava, Slovakia

    R. Staník & I. Benkovský

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  1. R. Staník
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  2. I. Benkovský
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Correspondence to R. Staník.

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Staník, R., Benkovský, I. 99mTc-labeling and molecular modeling of short dipeptide glycyl-l-proline. J Radioanal Nucl Chem 287, 949–953 (2011). https://doi.org/10.1007/s10967-010-0852-6

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  • DOI: https://doi.org/10.1007/s10967-010-0852-6

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