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Studies on 99mTc-labeling of the modified fragment of human alfa-fetoprotein (P149-QY)

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Abstract

The present study shows the development of 99mTc-labeling of the P149-QY peptide, a modified fragment of human alpha-fetoprotein (hAFP), with the intention of potential application for diagnosis of AFP positive tumors. An efficient preparation of 99mTc-labeled P149-QY using HYNIC was developed. It has been shown that the free sulfhydryl groups in P149-QY can be protected with N-ethylmaleimide, preventing the radiolabeled peptide from loss of its biological activity. The biodistribution studies revealed favorable pharmacokinetics and a better specificity to the mammary adenocarcinoma tumor for the SH-protected radiolabeled P149-QY peptide in comparison to the radioiodinated and to the directly 99mTc-labeled peptide.

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Authors and Affiliations

  1. Department of Radiopharmaceuticals, National Medicines Institute, 30/34 Chełmska Street, 00-725, Warsaw, Poland

    M. Maurin, P. Garnuszek & U. Karczmarczyk

  2. Department of Pharmaceutical Biochemistry, Molecular Biology Laboratory, Medical University, 1 Muszyńskiego Street, 90-151, Łódź, Poland

    M. Mirowski

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  1. M. Maurin
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  2. P. Garnuszek
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  3. U. Karczmarczyk
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  4. M. Mirowski
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Correspondence to M. Maurin.

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Maurin, M., Garnuszek, P., Karczmarczyk, U. et al. Studies on 99mTc-labeling of the modified fragment of human alfa-fetoprotein (P149-QY). J Radioanal Nucl Chem 275, 101–107 (2008). https://doi.org/10.1007/s10967-007-6980-y

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  • DOI: https://doi.org/10.1007/s10967-007-6980-y

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