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Resistance to induction of micronuclei, chromosomal aberrations and apoptosis by 60Co gamma-ray in a cell strain M5, derived from Chinese hamster V79 cells

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Abstract

The paper aims to investigate cytogenetic and apoptotic responses of γ-irradiation in a radio-resistant cell strain designated as M5. Induced micronuclei, chromosomal aberrations, nuclear fragmentation and nucleosomal ladders by γ-irradiation were less at equal doses in M5 cells in comparison with that obtained in the parental Chinese hamster V79 cells. However, at equal survival, there were no differences in the end points studied. Results indicate that the residual damages that lead to reproductive cell death also resulted in the cytogenetic and apoptotic responses. We speculate that the repair efficiency in M5 cells was more efficient and increased DNA repair could be the cause of radiation resistance observed in M5 cells.

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Authors and Affiliations

  1. Department of Biotechnology, West Bengal University of Technology, BF-142, Saltlake, Kolkata, 700 064, India

    R. Pathak & S. K. Dey

  2. Department of Zoology, Kalyani University, Kalyani, India

    A. R. Khuda Bukhsh

  3. Information Technology, B. P. Poddar Institute of Management and Technology, West Bengal, India

    B. Sen Gupta

  4. Army Referral Hospital, New Delhi, India

    M. Semwal

  5. Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700 064, India

    U. Ghosh & N. P. Bhattacharyya

Authors
  1. R. Pathak
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  2. A. R. Khuda Bukhsh
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  3. S. K. Dey
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  4. U. Ghosh
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  5. B. Sen Gupta
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  6. M. Semwal
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  7. N. P. Bhattacharyya
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Correspondence to N. P. Bhattacharyya.

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Pathak, R., Khuda Bukhsh, A.R., Dey, S.K. et al. Resistance to induction of micronuclei, chromosomal aberrations and apoptosis by 60Co gamma-ray in a cell strain M5, derived from Chinese hamster V79 cells. J Radioanal Nucl Chem 274, 441–447 (2007). https://doi.org/10.1007/s10967-007-1134-9

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  • DOI: https://doi.org/10.1007/s10967-007-1134-9

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