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Early response of lymphocyte proteins after gamma-radiation

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Abstract

In radiation biology there is a recent trend of looking for suitable biological parameters to detect early radiation-induced cellular damage. The present paper mentions 29 early response proteins (ERPRO) in human lymphocytes separated by 2 D-SDS-PA gelelectrophoresis 15 minutes after γ-radiation (2 Gy). A third of these proteins including phosphoproteins were identified using Nano-HPLC-MS. Most of the proteins were structural proteins (β-actin, mutant β-actin, talin and zyxin) except phosphoglycerate kinase-1, an enzyme of the glycolysis. For a more complete and detailed picture of ERPRO, the amount of proteins per gel has to be increased in the future by using a preparative gel instead of an analytical one.

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Authors and Affiliations

  1. Laboratory for Radiation Biology, Department of Safety and Radiation Protection, Research Centre Juelich, Juelich, D-52425, Germany

    A. Turtoi, D. Oskamp, R. Hille & F. H. A. Schneeweiss

  2. Chemistry Department, Panjab University, Chandigarh, 160014, India

    A. Srivastava

  3. Department of Biochemistry NEHU, Shillong, 793022, India

    R. N. Sharan

Authors
  1. A. Turtoi
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  2. A. Srivastava
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  3. R. N. Sharan
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  4. D. Oskamp
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  5. R. Hille
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  6. F. H. A. Schneeweiss
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Correspondence to A. Turtoi.

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Turtoi, A., Srivastava, A., Sharan, R.N. et al. Early response of lymphocyte proteins after gamma-radiation. J Radioanal Nucl Chem 274, 435–439 (2007). https://doi.org/10.1007/s10967-007-1133-x

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  • DOI: https://doi.org/10.1007/s10967-007-1133-x

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