Skip to main content
SpringerLink
Log in

Pharmacokinetics, tissue distribution and excretion of a recombinant fusion protein 125I-rhTNT-IL2

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

This study examined the tissues distribution and pharmacokinetics of rhTNT-IL2, a fusion protein, in rats and macaques after iv. injection. The rhTNT-IL2 was labeled with 125I. The 125I-rhTNT-IL2 was administered into rats and macaques at a dose of 250 μg·kg−1 and 125 μg·kg−1, respectively. Upon administration, rhTNT-IL2 declined in tri-exponentially with the half-lives of 1.78 h (T 1/2α), 25.06 h (T 1/2β), 114.19 h (T 1/2γ) for rats and 1.87 h (T 1/2α), 9.82 h (T 1/2β), 43.17 h (T 1/2γ) for macaques. The fraction of 125I-rhTNT-IL2 excreted in feces was low (about 1%), while the majority of 125I-rhTN-IL2 was excreted in urine. The tissue distributions showed that the liver, spleen and heart were major organs for deposition of the fusion protein in rats and macaques in 48-hour post-injection, and the fusion protein could not penetrate through blood brain barrier.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. T. A. Waldmann, S. Dubois, Y. Tagaya, Immunity, 14 (2001) 105.

    CAS  Google Scholar 

  2. T. A. Fehniger, M. A. Cooper, M. A. Caligiuri, Cytokine, 13 (2002) 169.

    CAS  Google Scholar 

  3. S. A. Rosenberg, Nature, 411 (2001) 380.

    Article  CAS  Google Scholar 

  4. P. M. Andson, E. Katsanis, S. F. Sencer et al., J. Immunother., 12 (1992) 19.

    Google Scholar 

  5. M. E. Neville, K. W. Richau, L. T. Boni et al., Cytokine, 12 (2000) 1702.

    Article  CAS  Google Scholar 

  6. K. Scheidhauer, I. Wolf, H.-J. Baumgartl et al., Eur. J. Nucl. Med. Mol. I, 29 (2002) 1276.

    Article  CAS  Google Scholar 

  7. Z.-P. Yolanda, L. S. Kegeles, Ningning Guo et al., Nucl. Med. Biol., 29 (2002) 575.

    Article  Google Scholar 

  8. Quanzhi Li, W. Hudson, Duo Wang et al., Cancer. Immunol. Immun., 47 (1998) 121.

    CAS  Google Scholar 

  9. E. S. Caldero N-Aranda, G. Riviere, V. Choumet et al., Toxicon, 37 (1999) 771.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Radiation Medicine and Public Health, Soochow University, 215123, Soochow, P.R. China

    Youjiu Zhang, Yujie Xu, Ran Zhu, Mingjiang Hu, Jianxiang Li, Yuejin Chen, Daojin Wang & Wo Fan

Authors
  1. Youjiu Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yujie Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ran Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mingjiang Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jianxiang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yuejin Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Daojin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Wo Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, Y., Xu, Y., Zhu, R. et al. Pharmacokinetics, tissue distribution and excretion of a recombinant fusion protein 125I-rhTNT-IL2. J Radioanal Nucl Chem 273, 3–8 (2007). https://doi.org/10.1007/s10967-007-0701-4

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-007-0701-4

Keywords

Search

Navigation