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Zinc uptake in swine intestinal brush border membrane vesicles using a 65Zn/69mZn duel isotope experiment

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Abstract

Supplemental zinc as ZnO is routinely added to nursery pig diets at 15 to 20 times the nutritional requirement to alleviate physiological stress and enhance performance. The mechanism by which Zn accomplishes this function is unknown, however, high concentrations of Zn are excreted in the environment at undesirable levels. To study Zn uptake, we developed a multi-stage digestion model followed by exposure to swine intestinal brush border membrane vesicles (BBMV). We report on the feasibility of using a duel label (65Zn and 69mZn) to simultaneously quantify the competitive uptake of Zn from co-existing zinc supplements using our BBMV model.

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Authors and Affiliations

  1. University of Missouri Research Reactor Center, Columbia, MO, 65211, USA

    C. E. Huntington & J. S. Morris

  2. Department of Animal Sciences, University of Missouri-Columbia, Columbia, MO, 65211, USA

    T. L. Veum

Authors
  1. C. E. Huntington
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  2. T. L. Veum
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  3. J. S. Morris
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Correspondence to C. E. Huntington.

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Huntington, C.E., Veum, T.L. & Morris, J.S. Zinc uptake in swine intestinal brush border membrane vesicles using a 65Zn/69mZn duel isotope experiment. J Radioanal Nucl Chem 276, 129–134 (2008). https://doi.org/10.1007/s10967-007-0421-9

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  • DOI: https://doi.org/10.1007/s10967-007-0421-9

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