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Antioxidant activity of bottlebrush copolymers and its self-assembly in mixed monolayers with cholesterol in the presence of cerium nitrate

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Abstract

The non-toxic amphiphilic bottlebrush polymers capable of self-organization on various interfaces and including cerium ions in structural assemblies without changing their biological properties have great importance for medicine and pharmacy. In the work, we studied the self-assembly in mixed monolayers of two copolymers – methoxy[oligo(propylene glycol)] methacrylate and methoxy[oligo(ethylene glycol)] methacrylate (PK) and its analogue with N-[3-(dimethylamino)propyl]methacrylamide) units quaternized with methyl iodide (PKq), and cholesterol on the aqueous subphase-air interface in the present of cerium compounds. The effect of the polymer-cerium composition on the oxidoruductase activity was investigated as well. The cationic polymer PKq was shown to exhibit higher specific activity of antioxidant enzymes than the non-ionic PK copolymer, increasing the activity of SOD by 20%, catalase – by 15%, GR – by 24%, G6PD – by 88%, and AlDH – by 10%. In the presence of cerium compounds (Ce(NO3)3, NH4[Ce(NO3)3], CeO2 nanoparticles) these effects increased significantly. Cholesterol in a monolayer of polymers contributed to an increase of compressibility modulus Cs−1 under the influence of cerium ions in mixed monolayers by approximately two times compared to mixed Chol+polymers monolayers on water, and Cs−1 became 640 mN/m for Chol+PK monolayers and 700 mN/m for Chol+PKq monolayers. AFM and wettability study suggest elastic structure of transferred mixed monolayers that is suitable for designing a drug delivery system.

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The datasets generated during the study are available from the corresponding author upon reasonable request.

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Funding

The study was financially supported by the Russian Science Foundation: synthesis and analyzes of nonionic bottlebrush copolymer was performed within the project No 23-73-00120, the rest of the researches were performed within the project No 22-73-10220.

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Authors and Affiliations

  1. The Faculty of Chemistry, the Lobachevsky State University, 23 the Gagarina Avenue, Nizhniy Novgorod, 603950, Russia

    Nina Melnikova & Olga Zamyshlyayeva

  2. Nizhny Novgorod State Technical University n.a. R.E. Alekseev, 24 Minin Street, Nizhniy Novgorod, 603950, Russia

    Nina Melnikova, Denis Kamorin, Oleg Kazantsev & Alexander Simagin

  3. Department of Pharmacy, Privolzhsky Research Medical University, 10/1 Minin Sq., Nizhniy Novgorod, 603950, Russia

    Alyona Sheferova

  4. The Razuvaev Institute of Organometallic Chemistry of the Russian Academy of Sciences, 49 the Tropinina Street, Nizhniy Novgorod, 603950, Russia

    Maxim Baten′kin

Authors
  1. Nina Melnikova
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  2. Olga Zamyshlyayeva
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  3. Denis Kamorin
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  4. Oleg Kazantsev
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  6. Alyona Sheferova
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  7. Maxim Baten′kin
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Contributions

Investigation, visualization, writing-original draft preparation, methodology N.M. and O.Z.; conceptualization, project administration N.M. and O.K.; synthesis and analysis of polymers D.K. and A.S.; obtaining surface pressure isotherms O.Z.; research by the method of AFM M.B.; wettability and biology activity A.B. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Nina Melnikova.

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Melnikova, N., Zamyshlyayeva, O., Kamorin, D. et al. Antioxidant activity of bottlebrush copolymers and its self-assembly in mixed monolayers with cholesterol in the presence of cerium nitrate. J Polym Res 31, 144 (2024). https://doi.org/10.1007/s10965-024-03999-1

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