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Hyaluronic acid-based self-repairing hydrogel preparation and 3D cell culture

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Abstract

Aldehyde-modified hyaluronic acid (Ald-HA) was prepared and characterized by a variety of methods, and then Ald-HA was used as hydrogel skeleton to fabricate self-healing hydrogel with biocompatible polyethylene glycol diacylhydrazide (PEG DH) as cross-linkers. For this purpose, aqueous solutions of Ald-HA and PEG DH solutions with different molecular weight were used to prepare HA-based hydrogel for potential applications in biological applications. This novel biodegradable hydrogel behaved with good mechanical properties and self-repairing performance. Moreover, the hydrogels have good porous structure fit for controlled release of doxorubicin (DOX∙HCl) and showed successive slow release profile. Importantly, CCK-8 assay demonstrated that the HA-based hydrogel possessed good cytocompatibility to JB6 P + cells. The 3D cell culture experiment reveals that the hydrogel acted as a good framework for cell growth and reproduction. These experiments indicate that the new HA-based hydrogel has excellent bio-compatibility for both preclinical and clinical applications.

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Funding

This research was funded by the Natural Science Foundation of China (No. 81601588, 82072327); Natural Science Foundation of Hebei Province (B2018201140); State key laboratory of organic–inorganic composites (oic-202001005); Medical Science Foundation of Hebei University(2021X01); Program of Excellent Innovative Talents in Hebei Provincial Institution of Higher Education (SLRC2017048); Hebei University (2017011, 2017014) and Post-graduate’s Innovation Fund Project of Hebei University (hbu2019ss010).

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Author notes

  1. Linmiao Zhu and Jiafu Shen are equally contributed to this work.

Authors and Affiliations

  1. Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases in Hebei Province, Hebei University, Baoding City, Hebei Province, 071002, China

    Linmiao Zhu, Wenjuan Li & Yong Wang

  2. College of Basic Medical, Hebei University, Baoding, 071002, Hebei Province, China

    Linmiao Zhu, Wenjuan Li & Yong Wang

  3. College of Chemistry and Environmental Science, Hebei University, Baoding, China

    Jiafu Shen, Heng An, Danyang Chen & Jianglei Qin

  4. State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, 100029, China

    Jianglei Qin

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  1. Linmiao Zhu
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  2. Jiafu Shen
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Correspondence to Yong Wang.

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Zhu, L., Shen, J., An, H. et al. Hyaluronic acid-based self-repairing hydrogel preparation and 3D cell culture. J Polym Res 29, 170 (2022). https://doi.org/10.1007/s10965-022-03017-2

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  • DOI: https://doi.org/10.1007/s10965-022-03017-2

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