Abstract
Pectin-graft-poly(2-acrylamido-2-methyl-1-propane sulfonic acid) (Pec-g-PAMPS) gel was made in the form of beads by subjecting the solution containing pectin (Pec), 2-acrylamido-2-methyl-1-propane sulfonic acid (AMPS) and ammonium peroxodisulphate to microwave irradiation followed by ionic crosslinking in CaCl2 solution. Gel beads containing silver nanoparticles were also prepared by the same method but with the addition of silver nitrate and trisodium citrate solution prior to microwave irradiation. The synthesized Pec-g-PAMPS and its silver nanocomposite (Pec-g-PAMPS-Ag) gel beads were characterized using FTIR, TGA, XRD, SEM, EDS and TEM techniques. The effect of incorporation of Ag NPs on the biological activity of Pec-g-PAMPS was studied by zone inhibition method considering two bacterial strains namely E. coli and B. subtilis. The nanocomposite gel exhibited higher antibacterial activity compared to the parent gel, which was comparable with the standard drug, Streptomycin. The in vitro drug release profiles of the parent gel and its composite were analyzed using Ketoprofen (KF) to study the effect of incorporation of Ag NPs on the drug release behavior of the Pec-g-PAMPS. The presence of silver nanoparticles enhanced both swelling of the gel beads and the extent of drug release significantly.
Similar content being viewed by others
References
Hamidi M, Azadi A, Rafiei P (2008) Adv Drug Deliv Rev 60:1638–1649
Khutoryanskiy VV (2007) Int J Pharm 33:415–426
Elvira C, Mano JF, San Román J, Reis RL (2002) Biomaterials 23:1955–1966
Siepmann J, Siegel RA, Rathbone MJ (eds) (2012) Fundamentals and applications of controlled release drug delivery, hydrogels (chapter 1). Springer, New York, pp 75–106
Coviello T, Matricardi P, Marianecci C, Alhaique FJ (2007) J Control Release 119:5–24
Nair LS, Laurencin CT (2007) Prog Polym Sci 32:762–798
Tian H, Tang Z, Zhuang X, Chen X, Jing X (2012) Prog Polym Sci 37:237–280
Suhag D, Bhatia R, Das S, Shakeel A, Ghosh A, Singh A, Sinha OP, Chakrabarti S, Mukherjee M (2015) RSC Adv 53:5963–53972
Kalia S, Sabaa MW (2013) Polysaccharide based graft copolymers. In: Kalia S, Sabaa MW, Kango S (eds) Polymer grafting: a versatile means to modify the polysaccharides (chapter-1). Springer-Verlag, Berlin, pp 1–14
de Souza JRR, de Carvalho JIX, Trevisan MTS, de Paula RCM, Ricardo NMPS, Feitosa JPA (2009) Food Hydrocoll 23:2278–2286
Sriamornsak P (1998) Int J Pharm 169:213–220
Itoh K, Hirayama T, Takahashi A, Kubo W, Miyazaki S, Dairaku M, Togashi M, Mikami R, Attwood D (2007) Int J Pharm 33:590–596
Munjeri O, Collett JH, Fell JTJ (1997) J Control Release 46:273–278
Sutar PB, Mishra RK, Pal K, Banthia AKJ (2008) J Mater Sci Mater Med 19:2247–2253
Junga J, Arnoldb RD, Wicker L (2013) Colloids Surf B: Biointerfaces 104:116–121
Dafe A, Etemadi H, Dilmaghani A, Mahdavinia GR (2017) Int J Biol Macromol 97:536–543
Suna X, Shi J, Xua X, Cao S (2013) Int J Biol Macromol 59:273–281
Hua S, Ma H, Li X, Yang H, Wang A (2010) Int J Biol Macromol 46:517–523
Zhang J, Wang Q, Wang A (2010) Acta Biomater 6:445–454
Pongjanyakul T, Puttipipatkhachorn S (2007) Int J Pharm 331:61–71
Zauro SA, Vishalakshi B (2018) Sep Sci Technol:1–17
Kodoth AK, Ghate VM, Lewis SA, Badalamoole V (2018) Int J Biol Macromol 115:418–430
Duran N, Duran M, de Jesus BM, Seabra AB, Favaro WJ, Nakazato G (2016) Nanomedicine: NBM 12:789–799
Guzman M, Dille J, Godet S (2012) Nanomedicine: NBM 8:37–45
Vimala K, Sivudu KS, Mohan YM, Sreedhar B, Raju KM (2009) Carbohydr Polym 75:463–471
Gulsonbi M, Parthasarathy S, Raj KB, Jaisankar V (2016) Ecotoxicol Environ Saf 134:421–426
Bardajee GR, Hooshyar Z, Kabiri F (2012) Bull Kor Chem Soc 33:2635–2641
Hooshyar Z, Bardajee GR (2017) J Iran Chem Soc 14:541–549
Gangadhar B, Vishalakshi B (2018) Polym Int. https://doi.org/10.1002/pi.5587
Siepmann J, Peppas NA (2011) Int J Pharm 418:6–12
Higuchi T (1963) J Pharm Sci 84:64–77
Hixsonl AW, Crowel JH (1931) Ind Eng Chem 23:923–931
Korsmeyer RW, Gurny R, Doelker EM, Buri P, Peppas NA (1983) Int J Pharm 15:25–35
Kabiri K, Mirzadeh H, Zohuriaan-Mehr MJ, Daliri M (2009) Polym Int 58:1252–1259 [27]
Mohan YM, Vimala K, Thomas V, Varaprasad K, Sreedhar B, Bajpai SK, Raju KM (2010) J Colloid Interface Sci 342:73–82
Jayaramudu T, Raghavendra GM, Varaprasad K, Sadiku R, Ramam K, Mohana Raju K (2013) Carbohydr Polym 95:188–194
Babu VR, Kim C, Kim S, Ahn C, Lee Y (2010) Carbohydr Polym 81:196–202
Akkaya MÇ, Emik S, Güçlü G, İyim TB, Özgümüş S (2009) J Appl Polym Sci 114:1150–1159
Sharma VK, Yngard RA, Lin Y (2009) Adv Colloid Interf Sci 145:83–96
Rai M, Yadav A, Gade A (2009) Biotechnol Adv 27:76–83
Li WR, Xie XB, Shi QS, Zeng HY, Yang YSO, Chen YB (2010) Appl Microbiol Biotechnol 85:1115–1122
Hörter D, Dressman JB (2001) Adv Drug Deliv Rev 46:75–87
Wang Q, Xi X, Zhang X, Zhang J, Wang A (2010) Int J Biol Macromol 46:356–362
Acknowledgements
The authors thank Mr. Naveen K, Research Scholar, Department of Biosciences, Mangalore University, Karnataka, India for carrying out the antibacterial studies.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Babaladimath, G., Badalamoole, V. Pectin-graft-poly(2-acrylamido-2-methyl-1-propane sulfonic acid) silver nanocomposite hydrogel beads: evaluation as matrix material for sustained release formulations of ketoprofen and antibacterial assay. J Polym Res 25, 202 (2018). https://doi.org/10.1007/s10965-018-1592-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s10965-018-1592-5