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In situ grafted nanostructured ZnO/carboxymethyl cellulose nanocomposites for efficient delivery of curcumin to cancer

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Abstract

In this present manuscript, zinc oxide (ZnO) nanoparticles embedded carboxymethyl cellulose (CMC) bionanocomposite were prepared by in situ grafting and the hydrophobic anticancer drug curcumin (Cur) was loaded into it. Structural, morphological, and physiochemical behavior of prepared curcumin-loaded CMC/ZnO nanocomposites (NCs) were characterized by FTIR, XRD, SEM, TEM, TGA, and DTA. The drug entrapment efficiency was evaluated and the in vitro efficacy as anticancer drug delivery vehicle was analyzed. The potential toxicity of curcumin-loaded ZnO/CMC NCs (Cur/ZnO/CMC NCs) was studied by using L929 and MA104 cell lines via MTT assay. The cellular uptake study of Cur/ZnO/CMC NCs by normal (L929) and cancer (MA104) cells carried out by using ethanol extraction and by FACS analysis has been reported. The results of this investigation demonstrate that the nanomatrix synthesized can effectively deliver the anticancer drug curcumin, and hence appears to be a promising nanoformulation for anticancer therapy and other biomedical applications.

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Acknowledgments

We thank Prof. P. Chakrabarti, Director, Motilal Nehru National Institute of Technology, Allahabad, India for providing other necessary facilities for research work. The author LU kindly acknowledges the Ministry of Human Resource Development (MHRD), Govt. of India for providing senior research fellowship (SRF) for research. JS kindly acknowledges Department of Science and Technology, New Delhi, India for awarding the INSPIRE FACULTY AWARD [IFA-13-CH-105].

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Authors and Affiliations

  1. Department of Applied Mechanics, MotiLal Nehru National Institute of Technology, Allahabad, 211004, India

    Laxmi Upadhyaya & R. P. Tewari

  2. Department of Biotechnology, MotiLal Nehru National Institute of Technology, Allahabad, 211004, India

    Vishnu Agarwal

  3. Department of Applied Chemistry, Delhi Technological University, Shahbad, Daulatpur, Main Bawana Road, Delhi, 110 042, India

    Jay Singh

  4. Nanotechnology Application Centre, University of Allahabad, Allahabad, 211002, India

    A.C. Pandey

  5. Centre for Genetic Disorders, Faculty of Science, Banaras Hindu University, Varanasi, 221 005, India

    Shiv P. Verma & Parimal Das

Authors
  1. Laxmi Upadhyaya
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  2. Jay Singh
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  4. A.C. Pandey
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  6. Parimal Das
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  7. R. P. Tewari
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Correspondence to Jay Singh.

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Upadhyaya, L., Singh, J., Agarwal, V. et al. In situ grafted nanostructured ZnO/carboxymethyl cellulose nanocomposites for efficient delivery of curcumin to cancer. J Polym Res 21, 550 (2014). https://doi.org/10.1007/s10965-014-0550-0

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  • DOI: https://doi.org/10.1007/s10965-014-0550-0

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