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Preparation and physicochemical properties of digested collagen fragments with varying molecular weights

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Abstract

The porcine dermal collagen molecules were successfully digested by clostridium histolyticun collagenases (CHC) and then ultra-filtrated using varying grades of ultra-filtration membranes. Gel permeation analyses revealed that collagen fragments with varying molecular weights were successfully segregated using ultra-filtration membranes with varying grades of pore sizes. Fourier transform infra-red analyses suggest that digested collagen fragments and digested collagen fragments prepared after ultra-filtration still preserve certain percentages of triple helix structures of collagen molecules, although the percentages of preserved triple helix structures present in digested and ultra-filtrated collagen fragments reduce significantly as their Mw values reduce. Thermal and denaturation temperature analysis suggest that denaturation temperature and thermal degradation temperature values of digested collagen fragments and ultra-filtrated collagen fragments decrease significantly as their Mw values reduce. The absorbed/desorbed amounts of digested and ultra-filtrated collagen fragments in PA6/PP flocking specimens are significantly higher than those of the original collagens, and increase significantly as their Mw values reduce. Possible reasons accounting for the above degradation, ultra-filtration physicochemical, absorbing and desorbing properties of original, digested and ultra-filtrated digested collagen molecules are reported.

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Acknowledgements

The authors would like to express their appreciation to the Department of Industrial Technology, Ministry of Economic Affairs (95-EC-17-A-11-S1-057, 96-EC-17-A-11-S1-057, 97-EC-17-A-11-S1-057 and 99-EC-17-A-11-S1-155) for support of this work.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan

    Jen-taut Yeh, Haw-jer Chang & Chi-hui Tsou

  2. Ministry of Education, Key Laboratory for the Green Preparation and Application of Functional Materials, Faculty of Materials Science and Engineering, Hubei University, Wuhan, China

    Jen-taut Yeh & Li Yang

  3. Key Laboratory of Green Processing and Functional Textiles of New Textile Materials (Wuhan University of Science and Engineering), Ministry of Education, Wuhan, China

    Jen-taut Yeh & Wei Wei

  4. School of Printing and Packaging, Wuhan University, Wuhan, China

    Jen-taut Yeh

  5. Department of Materials Engineering, Kun Shan University, Tainan, Taiwan

    Jen-taut Yeh

  6. Department of Materials and Textiles, Oriental Institute of Technology, Taipei, Taiwan

    Wei-hua Yao

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  1. Jen-taut Yeh
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  2. Haw-jer Chang
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  3. Li Yang
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  4. Chi-hui Tsou
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  5. Wei Wei
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  6. Wei-hua Yao
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Correspondence to Jen-taut Yeh or Wei-hua Yao.

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Yeh, Jt., Chang, Hj., Yang, L. et al. Preparation and physicochemical properties of digested collagen fragments with varying molecular weights. J Polym Res 19, 26 (2012). https://doi.org/10.1007/s10965-012-0026-z

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  • DOI: https://doi.org/10.1007/s10965-012-0026-z

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