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Properties of a Calcium Phosphate Cement Synergistically Reinforced by Chitosan Fiber and Gelatin

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Abstract

Calcium phosphate cement (CPC) composite by the incorporation of chitosan fiber (CF) and gelatin was investigated for flexural strength, phase composition, microstructure, and in vitro cellular response. The results demonstrated that the incorporation improved the flexural strength significantly (maximum, 12.31 MPa), together with accelerating the conversion of CPC components to hydroxyapatite (HA). Rat bone marrow stromal cells (RBMSCs) spread and grew actively on the composite discs. Short-term minimum essential medium (MEM) extraction tests showed no cell growth inhibition. These results indicated that the new composite not only had a reinforced flexural property, but it was also beneficial for RBMSC growth.

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Abbreviations

CPC:

Calcium phosphate cement

CS:

Chitosan

CF:

Chitosan fiber

RBMSCs:

Rat bone marrow stromal cells

TTCP:

Tetracalcium phosphate

DCPA:

Dicalcium phosphate anhydrous

XRD:

X-ray diffraction

SEM:

Scanning electron microscopy

DMEM:

Dulbecco's modified Eagle medium

FBS:

Fetal bovine serum

PBS:

Phosphate-buffered saline

GFP:

Green fluorescence protein

TCPS:

Tissue culture polystyrene

MEM:

Minimum essential medium

MTT:

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

DMSO:

Dimethyl sulfoxide

OD:

Optical density

ANOVA:

One-way analysis of variance

HA:

Hydroxyapatite

JCPDS:

Joint committee on powder diffraction standards

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Authors and Affiliations

  1. Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710038, People's Republic of China

    Zhao Hui Pan & Qing Yu Fan

  2. State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710032, People's Republic of China

    He Ping Cai

  3. Orthopedics Institute of Chinese PLA, 89th Hospital, Weifang, Shandong Province, 261000, People's Republic of China

    Ping Ping Jiang

Authors
  1. Zhao Hui Pan
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  2. He Ping Cai
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  3. Ping Ping Jiang
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  4. Qing Yu Fan
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Correspondence to Qing Yu Fan.

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Pan, Z.H., Cai, H.P., Jiang, P.P. et al. Properties of a Calcium Phosphate Cement Synergistically Reinforced by Chitosan Fiber and Gelatin. J Polym Res 13, 323–327 (2006). https://doi.org/10.1007/s10965-006-9041-2

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  • DOI: https://doi.org/10.1007/s10965-006-9041-2

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