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The Effect of Dimethylsulfoxide (or Diethylsulfoxide) on Methylene Blue-Calf Thymus DNA Binding in Aqueous Solutions by Fluorescence Polarization and Steady-State Fluorescence Quenching

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Abstract

The detailed study of the effect of dimethylsulfoxide (or diethylsulfoxide) on interaction between methylene blue (MB) and calf thymus DNA was performed at 293.15 K using steady-state fluorescence quenching and fluorescence polarization. The combination of steady-state fluorescence quenching and fluorescence polarization reveal the main binding modes at different concentrations of DNA and the effect of sulfoxides on binding mechanism. For comparison, the other polar solvents such as N,N-dimethyl formamide and acetonitrile were used. From Stern–Volmer equation, the values of binding constant and standard Gibbs free energy change were determined. At low concentrations of DNA with addition of various organic cosolvents, the binding constant of DNA–MB complex decreases indicating that hydrogen bonds are predominant, and thus, the main binding mode is groove binding. At high concentrations of DNA, the binding mechanism changes and intercalation becomes the main binding mode in all solutions. Addition of cosolvents distorts the groove binding and, as a result, weakens the intercalation between MB and base pairs of double-stranded DNA.

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Acknowledgements

The work was supported by the Science Committee of RA, in the frames of the research project № 21T‐1D026.

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Authors and Affiliations

  1. Deparment of Inorganic and Analytical Chemistry, Yerevan State University, 0025, Yerevan, Armenia

    Gohar A. Shahinyan

  2. Department of Physical and Colloid Chemistry, Yerevan State University, 0025, Yerevan, Armenia

    Heghine H. Ghazoyan & Shiraz A. Markarian

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  1. Gohar A. Shahinyan
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  2. Heghine H. Ghazoyan
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  3. Shiraz A. Markarian
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SAM contributed to the objectives of the problems and explanation of obtained results. HHG contributed to explanation of obtained results. GAS contributed to experiments and explanation of obtained results. All authors reviewed the manuscript.

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Correspondence to Shiraz A. Markarian.

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Shahinyan, G.A., Ghazoyan, H.H. & Markarian, S.A. The Effect of Dimethylsulfoxide (or Diethylsulfoxide) on Methylene Blue-Calf Thymus DNA Binding in Aqueous Solutions by Fluorescence Polarization and Steady-State Fluorescence Quenching. J Solution Chem 52, 708–719 (2023). https://doi.org/10.1007/s10953-023-01263-6

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