Abstract
Preferential interactions of α-chymotrypsinogen in water and aqueous solutions of ethylene glycol, glycerol, erythritol, sorbitol, and inositol were studied from precise density measurements under conditions of constant molality and constant chemical potential; to achieve the last condition, solutions were brought under equilibrium dialysis. The density measurements were performed at 298.15 K and they were used to determine the preferential interaction parameters. The results confirm a significant correlation between the numbers of hydroxyl groups of the polyols with the stability of α-chymotrypsinogen.
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Acknowledgements
This work was supported by Universidad Nacional de Colombia, and the National Program of Researches Training 2012 COLCIENCIAS.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were made by Juan Sebastian Abella and Carmen Maria Romero. The first draft of the manuscript was written by Juan Sebastian Abella and both authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Abella, J.S., Romero, C.M. Preferential Interaction of Chymotrypsinogen in Aqueous Solutions of Polyols at 298.15 K. J Solution Chem 48, 1591–1602 (2019). https://doi.org/10.1007/s10953-019-00936-5
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DOI: https://doi.org/10.1007/s10953-019-00936-5