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Fixed-Dose Combination Antibiotics Interacting with a Quaternary Ammonium Disinfectant: Insights from Spectral and Chromatographic Measurements

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Abstract

Core–shell architecture of surfactant micelles can act to shield active drug molecules from the adverse environmental conditions and enhance their bioavailability. In the present study the molecular interactions of a fixed dose combination (FDC) containing ofloxacin (OFX) and ornidazole (ORN), with cetyltrimethylammonium bromide (CTAB, a quaternary ammonium surfactant) were investigated under normal physiological pH (pH 7.4 ± 0.1). The impact of the cationic micelle on the spectral and physiochemical properties of the FDC components OFX and ORN was systematically examined by electronic spectroscopy. Micellar liquid chromatography and differential spectral methods were used to optimize analysis of the magnitude of binding constants and related Gibbs energies. The results suggest the potential solubilization of drugs in the external part of the micelles, which may prove to be more helpful for their controlled release. These outcomes have been verified by the binding capacities of drug–CTAB combinational system that may be helpful to customize the uptake of drug molecules in cells.

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Acknowledgements

The authors express their gratitude to the Chemistry Department, Government College University, Lahore, Pakistan for provision of laboratory facilities. The authors also extend their sincere appreciations to the Higher Education Commission of Pakistan for providing financial support through NRPU Project No. 4557.

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Authors and Affiliations

  1. Department of Chemistry, Government College University, Lahore, 54000, Pakistan

    Waqar Azeem, Peter John, Islam Ullah Khan & Shahzad Sharif

  2. Department of Chemistry, University of Gujrat, Gujrat, 50700, Pakistan

    Muhammad Faizan Nazar & Muhammad Ashfaq

  3. Lahore Chemical & Pharmaceutical Works (Pvt.) Ltd, Lahore, 54590, Pakistan

    Atif Riaz

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  1. Waqar Azeem
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  2. Peter John
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  3. Muhammad Faizan Nazar
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  4. Muhammad Ashfaq
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Azeem, W., John, P., Nazar, M.F. et al. Fixed-Dose Combination Antibiotics Interacting with a Quaternary Ammonium Disinfectant: Insights from Spectral and Chromatographic Measurements. J Solution Chem 47, 1048–1059 (2018). https://doi.org/10.1007/s10953-018-0773-5

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