Journal of Solution Chemistry

, Volume 47, Issue 3, pp 586–594 | Cite as

Interactions of Bovine Serum Albumin Molecules in an Aqueous Sodium Sulfate Solution Determined by an Osmotic Pressure Method

  • Gongke Wang
  • Shuangli Wang
  • Hongwei Wu
  • Changling Yan
  • Yufang Liu


In this work, the interactions of aqueous bovine serum albumin (BSA) solutions at four pH values (4.5, 4.8, 5.4, and 7.4), and at three sodium sulfate (Na2SO4) concentrations (0.15, 0.50, and 1.00 mol·L−1), was investigated through the use of osmotic pressure measurements. The osmotic second virial coefficients of BSA were determined from the osmotic pressure data. According to the molecular thermodynamic model, as described in the literature (Moon et al. in Fluid Phase Equilib 168:229–239, 2000), the electrostatic repulsion potential, attractive dispersion potential and the ion-excluded-volume potential were calculated. The dependencies of the three potentials on the solution pH and on the ionic strength are discussed. We compared the potentials of BSA molecules in an aqueous sodium sulfate solution with the potentials in aqueous ammonium sulfate [(NH4)2SO4] and sodium chloride (NaCl) solutions. The order of the potential values in the aqueous solutions with the different salts was also discussed.

Graphical Abstract


Bovine serum albumin Osmotic pressure Osmotic second virial coefficient Interaction 



This work was supported by the Program for Innovative Research Team in University of Henan Province (No. 18IRTSTHN003), the China Postdoctoral Science Foundation (No. 2016M592292), the National Natural Science Foundation of China (No. 21303043), the Key Scientific Research Project of Higher Schools in Henan Province (No. 14A150012), Science and Technology Innovation Talent Project of Xinxiang, China (No. CXRC16005) and the Key Technologies R & D Program of Xinxiang, China (No. CXGG16006).

Supplementary material

10953_2018_738_MOESM1_ESM.doc (134 kb)
Electronic supplementary material 1 (DOC 134 kb)


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Authors and Affiliations

  1. 1.School of Chemistry and Chemical Engineering, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine ChemicalsHenan Normal UniversityXinxiangPeople’s Republic of China
  2. 2.School of Physics and Materials ScienceHenan Normal UniversityXinxiangPeople’s Republic of China

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