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Journal of Solution Chemistry

, Volume 46, Issue 4, pp 896–907 | Cite as

Liquid Chromatography Tandem Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy of Magnesium (II) Gluconate Solution

  • Mahendra Kumar Trivedi
  • Parthasarathi Panda
  • Kalyan Kumar Sethi
  • Snehasis Jana
Article
  • 151 Downloads

Abstract

Magnesium gluconate is a classical pharmaceutical compound used as a source of magnesium for the prevention and treatment of hypomagnesemia. To the best of our knowledge, a robust and reliable liquid chromatography tandem mass spectrometry technique has not yet been reported for the qualitative and quantitative analysis of magnesium gluconate. This study describes the method development for the LC–ESI–MS/MS analysis of magnesium gluconate using three different reversed-phase HPLC conditions (Method I–III) with comprehensive fragmentation pattern and the structural characterization by NMR spectroscopy. The LC–MS and NMR data were found in accordance with the structure of magnesium gluconate. When magnesium gluconate was dissolved in the acetonitrile and water–methanol solutions, it exists in situ in three different forms: magnesium gluconate itself, gluconic acid, and magnesium gluconate chelate with gluconic acid by a coordinate covalent bond. Method I exhibited pseudo-molecular ion peaks with more magnesium gluconate chelates with gluconic acid, while method II showed an adduct of magnesium gluconate with the solvent along with the molecular ion peak. There was no pseudo-molecular ion peaks found in method III. Thus, method III was found to be the more accurate, robust and reliable LC–MS method for the qualitative and quantitative analysis, structural characterization, and could also be suitable for the pharmacokinetic study of magnesium gluconate. The detailed fragmentation analysis might be useful for the structural characterization of unknown divalent organometallics.

Keywords

Magnesium gluconate Liquid chromatography tandem mass spectrometry Nuclear magnetic resonance spectroscopy Organometallic Electrospray ionization 

Notes

Acknowledgements

The authors are grateful to GVK Biosciences Pvt. Ltd., Hyderabad, India for their assistance and support during this work.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Mahendra Kumar Trivedi
    • 1
  • Parthasarathi Panda
    • 2
  • Kalyan Kumar Sethi
    • 2
  • Snehasis Jana
    • 2
  1. 1.Trivedi Global Inc.HendersonUSA
  2. 2.Trivedi Science Research Laboratory Pvt. Ltd.,BhopalIndia

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