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Liquid–Liquid Equilibria of Water + 1-Butanol + Amino Acid (Glycine or dl-Alanine or l-Leucine) at 313.15 K

Journal of Solution Chemistry volume 43, pages 2101–2116 (2014)Cite this article

Abstract

Amino acids play an important role both in animal metabolism and in industrial processes. Since they are rarely found in nature in a free form, they must be obtained by biosynthesis or protein hydrolysis, often resulting in aqueous mixtures containing various solutes including several types of amino acids. As a consequence, the cost of the separation processes and concentration of amino acids can be as high as 90 % of their total manufacturing cost. In this way, the design of such processes requires knowledge of the partitioning behavior in two-phase systems and thermodynamic models should support this optimization procedure. However, both the complexity of biomolecules, due to their multiple functional groups, and the crucial role of water make the standard available thermodynamic models unattractive for the computation of phase behavior. Therefore, new thermodynamic models for the description of the phase behavior of systems containing biomolecules are required. In this work, liquid–liquid equilibrium data of the ternary systems 1-butanol + water + amino acid (glycine, dl-alanine or l-leucine) were measured at 313.15 K. The experimental results were correlated and interaction parameters were estimated for the modified-NRTL, UNIFAC and UNIFAC-Campinas models. The modified-NRTL model was able to describe the data better than the UNIFAC and UNIFAC-Campinas models.

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Acknowledgments

The authors acknowledge Dr. E. A. Macedo (University of Porto, Portugal) and Dr. S. P. Pinho (currently at Salvador University, Brazil) for their experimental support and useful theoretical discussions. Dr. G. R. Santos acknowledges the financial support from Program Alβan—European Union Program of High Level Scholarships for Latin America, process E03D09468BR. Dr. M. Aznar is the recipient of a CNPq research fellowship.

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Authors and Affiliations

  1. School of Chemical Engineering, Federal University of Pará, Campus do Guamá, Belém, PA, 66000-000, Brazil

    Geormenny R. Santos & Danilo C. Souza

  2. School of Chemical Engineering, University of Campinas, Av. Albert Einstein 500, Campinas, SP, 13083-852, Brazil

    Martín Aznar

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  1. Geormenny R. Santos
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Santos, G.R., Souza, D.C. & Aznar, M. Liquid–Liquid Equilibria of Water + 1-Butanol + Amino Acid (Glycine or dl-Alanine or l-Leucine) at 313.15 K. J Solution Chem 43, 2101–2116 (2014). https://doi.org/10.1007/s10953-014-0265-1

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  • DOI: https://doi.org/10.1007/s10953-014-0265-1

Keywords

  • Liquid–liquid equilibrium
  • Amino acid
  • Alcohol
  • Partition coefficient
  • Modeling