Abstract
In this study, we report the pH values of two buffer solutions without chloride ion and eight buffer solutions with NaCl with an ionic strength I=0.16 mol⋅kg−1. Electromotive force (emf) techniques have been used to get the cell potentials at 12 temperatures from 5 to 55 °C, including 37 °C. An extended form of the Bates-Guggenheim convention is used in the entire ionic strength range, 0.04 to 0.16 mol⋅kg−1. The residual liquid junction potentials (δE j ) of the buffer solutions of MOBS have been estimated from previous measurements with a flowing junction cell. These values of δE j have been used for correction in order to ascertain the operational pH values of four buffer solutions of MOBS at 25 and 37 °C. These solutions are recommended as pH standards for physiological application in the pH range 7.4 to 7.7.
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References
Good, N.E., Winget, G.D., Winter, W., Connolly, T.N., Izawa, S., Singh, R.M.M.: Hydrogen ion buffers for biological research. Biochem. 5, 467–477 (1966)
Ferguson, W.J., Braunschweiger, K.I., Braunschweiger, W.R., Smith, J.R., McCormick, J.J., Wasmann, C.C., Jarvis, N.P., Bell, D.H., Good, N.E.: Hydrogen ion buffers for biological research. Biochem. 104, 300–310 (1980)
Roy, R.N., Roy, L.N., Grant, J.G., Cummins, M.P., Tabor III, B.J., Richards, S.J., Himes, C.A., Lively, B.R., Blackwell, P.L., Simon, A.N.: Second dissociation constants of 4-[N-morpholino]butanesulfonic acid and N-[2-hydroxyethyl]piperazine-N′-4-butanesulfonic acid from 5 to 55 °C. J. Solution Chem. 31, 861–872 (2002)
Vega, C.A., Bates, R.G.: Buffers for the physiological pH range: thermodynamic constants of four substituted aminoethanesulfonic acid from 5 to 50 °C. Anal. Chem. 48, 1293–1296 (1976)
Bower, V.E., Paabo, M., Bates, R.G.: A. standard for the measurement of the pH of blood and other physiological media. J. Res. Natl. Bur. Stand. 65A, 267–270 (1961)
Durst, R.A., Staples, B.R.: Tris/Tris HCl: standard buffer for use in the physiological pH range. Clin. Chem. 18, 206–208 (1972)
Roy, L.N., Roy, R.N., Denton, C.E., LeNoue, S.R., Roy, C.N., Ashkenazi, S., Williams, T.B., Church, D.R., Fuge, M.S., Sreepada, K.S.: Second dissociation constant of bis-[2-hydroxyethyl)amino]acetic acid (BICINE) and pH of its buffer solutions from 5–55 °C. J. Solution Chem. 35, 605–624 (2006)
Feng, D., Koch, W.F., Wu, Y.C.: Second dissociation constant and pH of N-(2-hydroxyethyl)piperazine-N′-2-ethanesulfonic acid from 0 to 50 °C. Anal. Chem. 61, 1400–1405 (1989)
Roy, R.N., Mrad, D.R., Lord, P.A., Carlsten, J.A., Good, W.S., Allsup, P., Roy, L.N., Kuhler, K.M., Koch, W.F., Wu, Y.C.: Thermodynamics of the second dissociation constant and standards for pH of 3-(N-morpholino)propanesulfonic acid (MOPS) from 5 to 55 °C. J. Solution Chem. 27, 73–87 (1998)
Wu, Y.C., Berezansky, P.A., Feng, D., Koch, W.F.: Second dissociation constant of 3-(N-morpholino)-2-hydroxypropanesulfonic acid and pH of its buffer solutions. Anal. Chem. 65, 1084–1087 (1993)
Bates, R.G., Roy, R.N., Robinson, R.A.: Buffer standards of tris(hydoxymethyl)methylglycine (“tricine”) for the physiological range pH 7.2 to 8.5. Anal. Chem. 45, 1663–1666 (1973)
Goldberg, R.N., Kishore, N., Lennen, R.M.: Thermodynamic quantities for the ionization reactions of buffers. J. Phys. Chem. Ref. Data 31, 231–370 (2002)
Bates, R.G.: Determination of pH, 2nd edn. Wiley, New York (1973)
Bates, R.G.: Revised standard values for pH measurements from 0 to 95 °C. J. Res. Natl. Bur. Stand. 66A, 179–184 (1962)
Latimer, W.M.: Oxidation Potentials, 2nd edn. Prentice Hall, New York (1952)
Bates, R.G., Vega, C.A., White, D.R. Jr.: Standards for pH measurements in isotonic saline media of ionic strength I=0.16. Anal. Chem. 50, 1295–1300 (1978)
Partanen, J.I., Minkkinen, P.O.: Redetermination of the second dissociation constant of phosphoric acid and calculation of the pH values of the pH standards based on solutions of dihydrogen and hydrogen phosphate ions at 25 °C. Acta Chem. Scand. 50, 1081–1086 (1996)
Covington, A.K., Ferra, M.I.A.: A Pitzer mixed electrolyte solution theory approach to assignment of pH to standard buffer solutions. J. Solution Chem. 23, 1–10 (1994)
Buck, R.P., Rondinini, S., Covington, A.K., Baucke, F.G.K., Brett, C.M.A., Camoes, M.F., Milton, M.J.T., Mussini, T., Naumann, R., Pratt, K.W., Spitzer, P., Wilson, G.S.: Measurement of pH, definition, standards, and procedures. Pure Appl. Chem. 74, 2169–2200 (2002)
Roy, R.N., Gibbons, J.J., Williams, R., Godwin, L., Baker, G., Simonson, J.M., Pitzer, K.S.: The thermodynamics of aqueous carbonate solutions. II. Mixtures of potassium carbonate, bicarbonate, and chloride. J. Chem. Thermodyn. 16, 303–315 (1984)
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The authors are grateful for the funding from the National Institutes of Health (AREA), under the grant 2-R15 GM 066866-03 and the diversity supplemental grant 3-R15 GM 066866-03 S1. The authors would also like to thank Daniel McPherson and Ishita Pareek for their dedicated and hard work. The content of this paper is the sole responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or the National Institutes of General Medical Sciences.
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Roy, R.N., Roy, L.N., Allen, K.A. et al. Buffers for the Physiological pH Range: Studies of 4-(N-Morpholino)butanesulfonic Acid (MOBS) from 5 to 55 °C. J Solution Chem 41, 1044–1053 (2012). https://doi.org/10.1007/s10953-012-9847-y
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DOI: https://doi.org/10.1007/s10953-012-9847-y