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Study on the Synergism Effect of Lomefloxacin and Ofloxacin for Bovine Serum Albumin in Solution by Spectroscopic Techniques

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Abstract

Both lomefloxacin (LOM) and ofloxacin (OFL) have a powerful ability to quench the fluorescence of bovine serum albumin (BSA). The fluorescence quenching action is much stronger when the two drugs coexist. The synergism between LOM and OFL was studied using fluorescence and ultraviolet spectroscopy under imitated physiological conditions. The results show that static quenching and non-radiation energy transfer are the main reasons for the fluorescence quenching. The synergism results in both the reduction of the binding stability between drugs and BSA and an increase of the free drug concentration, which will increase the efficacy of drugs. The thermodynamic parameters at different temperatures were calculated and the binding distances r between the drugs and BSA were obtained based on Försters theory of non-radiation energy transfer. The synchronous fluorescence spectra indicated that the effect of synergism affected the conformation of BSA.

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Authors and Affiliations

  1. Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, 411201, China

    Xianyong Yu, Ronghua Liu, Danhong Ji, Fengxian Yang, Xiaofang Li, Jiming Zhou & Pinggui Yi

  2. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA, 92037, USA

    Jian Xie

Authors
  1. Xianyong Yu
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  2. Ronghua Liu
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  3. Danhong Ji
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  4. Fengxian Yang
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  5. Xiaofang Li
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  6. Jian Xie
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  7. Jiming Zhou
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  8. Pinggui Yi
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Correspondence to Xiaofang Li or Pinggui Yi.

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Yu, X., Liu, R., Ji, D. et al. Study on the Synergism Effect of Lomefloxacin and Ofloxacin for Bovine Serum Albumin in Solution by Spectroscopic Techniques. J Solution Chem 40, 521–531 (2011). https://doi.org/10.1007/s10953-011-9653-y

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  • DOI: https://doi.org/10.1007/s10953-011-9653-y

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