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Thermodynamic Study on the Interaction of Copper Ion with Human Growth Hormone

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Abstract

A thermodynamic study on the interaction between the Cu2+ ion and human growth hormone (hGH) was studied at the temperatures 300.15 and 310.15 K in NaCl solution using isothermal titration calorimetry. The new solvation model was used to reproduce the enthalpies of Cu2++hGH interaction over the whole range of Cu2+ concentrations. It was found that there is a set of three identical and non-interacting binding sites for Cu2+ ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 1313.4 μmol⋅L−1 and −16.80 kJ⋅mol−1 at 300.15 K, and 1648.2 μmol⋅L−1 and −16.40 kJ⋅mol−1 and 310.15 K, respectively. The binding parameters recovered from the new equation are attributed to a structural change of hGH and its biological activity due to metal ion interaction.

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Authors and Affiliations

  1. Department of Chemistry, Imam Khomeini International University, Qazvin, Iran

    G. Rezaei Behbehani

  2. Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran

    G. Rezaei Behbehani, E. Tazikeh & M. Monajjemi

  3. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    A. A. Saboury & A. Divsalar

  4. Department of Biological Sciences, Tarbiat Moallem University, Tehran, Iran

    A. Divsalar

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  1. G. Rezaei Behbehani
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  2. E. Tazikeh
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  3. A. A. Saboury
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  4. A. Divsalar
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  5. M. Monajjemi
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Correspondence to G. Rezaei Behbehani.

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Rezaei Behbehani, G., Tazikeh, E., Saboury, A.A. et al. Thermodynamic Study on the Interaction of Copper Ion with Human Growth Hormone. J Solution Chem 39, 153–164 (2010). https://doi.org/10.1007/s10953-010-9498-9

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  • DOI: https://doi.org/10.1007/s10953-010-9498-9

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