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Measurement of Magnesium Stability Constants of Biologically Relevant Ligands by Simultaneous Use of pH and Ion-Selective Electrodes

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Abstract

Simultaneous measurements with magnesium ion-selective and pH glass electrodes have been used for determination of the stability constants of magnesium ions with various biologically relevant ligands by alkalimetric titration under physiological conditions (37 ○C, I=0.15 mol⋅dm−3). New systems were investigated: magnesium with pyroglutamate, pyridoxine and HEPES, along with citrate, lactate, glycinate, aspartate and glutamate. For comparison, calcium stability constants with the same ligands were determined similarly, using calcium ion-selective and pH glass electrodes. Ligand protonation constants, necessary for the calculation of the metal complex formation constants, were also determined.

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  1. E. Y. Danish

    Present address: Department of Chemistry, King Abdul-Aziz University, Jeddah, Saudi Arabia

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  1. Department of Chemistry, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK

    A. K. Covington & E. Y. Danish

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  1. A. K. Covington
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  2. E. Y. Danish
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Correspondence to A. K. Covington.

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Covington, A.K., Danish, E.Y. Measurement of Magnesium Stability Constants of Biologically Relevant Ligands by Simultaneous Use of pH and Ion-Selective Electrodes. J Solution Chem 38, 1449–1462 (2009). https://doi.org/10.1007/s10953-009-9459-3

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  • DOI: https://doi.org/10.1007/s10953-009-9459-3

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