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An Alternate Solution of Fluorescence Recovery Kinetics after Spot-Bleaching for Measuring Diffusion Coefficients. 2. Diffusion of Fluorescein in Aqueous Sucrose Solutions

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Abstract

The traditional analysis of the fluorescence recovery kinetics after spot bleaching yields expressions for the diffusion coefficient of the probe that are not suitable for linear fittings. In a previous work we developed an improved recovery function that is a better alternative for data analysis. To illustrate its application to real cases and compare it with the previous data treatment, we measured the time response of fluorescein in aqueous sucrose solutions, covering the unsaturated and the supercooled region, where decoupling between diffusion and viscosity is observed. The results are compared with the mobility of different types of solutes in aqueous sucrose solutions and are discussed in terms of the classical hydrodynamic model.

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Author notes

  1. M. C. Marconi

    Present address: NSF ERC for Extreme Ultraviolet Science and Technology and Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, 80525, USA

Authors and Affiliations

  1. Departamento de Fisica de Materia Condensada, Centro Atómico Constituyentes, Comisión Nacional de Energía Atómica, San Martin, Buenos Aires, 1650, Argentina

    H. R. Corti

  2. Instituto de Química Física de los Materiales, Ambiente y Energía (INQUIMAE), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina

    H. R. Corti

  3. Laboratorio de Electrónica Cuántica, Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina

    G. A. Frank & M. C. Marconi

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  1. H. R. Corti
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Correspondence to H. R. Corti.

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Corti, H.R., Frank, G.A. & Marconi, M.C. An Alternate Solution of Fluorescence Recovery Kinetics after Spot-Bleaching for Measuring Diffusion Coefficients. 2. Diffusion of Fluorescein in Aqueous Sucrose Solutions. J Solution Chem 37, 1593–1608 (2008). https://doi.org/10.1007/s10953-008-9329-4

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