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A Comparative Study of the Direct Calorimetric Determination of the Denaturation Enthalpy for Lysozyme in Sodium Dodecyl Sulfate and Dodecyltrimethylammonium Bromide Solutions

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Abstract

The complexes of lysozyme with the anionic surfactant sodium dodecyl sulfate (SDS) and the cationic surfactant dodecyltrimethylammonium bromide (DTAB) have been investigated by isothermal titration calorimetry at pH=7.0 and 27 °C in a phosphate buffer. A new direct calorimetric method was applied to follow the protein denaturation and study the effect of surfactants on the stability of proteins. The extended solvation model was used to represent the enthalpies of lysozyme + SDS interaction over the whole range of SDS concentrations. The solvation parameters recovered from the new equation are attributed to the structural change of lysozyme and its biological activity. At low SDS concentrations, the binding is mainly electrostatic with some simultaneous interaction of the hydrophobic tail with nearby hydrophobic regions of lysozyme. These initial interactions presumably cause some protein unfolding and expose additional hydrophobic sites. The induced enthalpy of denaturation of lysozyme by SDS is 160.81±0.02 kJ⋅mol−1. The lysozyme-DTAB complexes behave very differently from those of the lysozyme-SDS complexes. SDS induces a stronger unfolding of lysozyme than DTAB. The induced enthalpy of lysozyme denaturation by DTAB is 86.46±0.02 kJ⋅mol−1.

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Authors and Affiliations

  1. Chemistry Department, Imam Khomeini International University, Nooroozian st., Qazvin, Iran

    G. Rezaei Behbehani & E. Taleshi

  2. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    A. A. Saboury

Authors
  1. G. Rezaei Behbehani
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  2. A. A. Saboury
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  3. E. Taleshi
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Correspondence to G. Rezaei Behbehani.

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Rezaei Behbehani, G., Saboury, A.A. & Taleshi, E. A Comparative Study of the Direct Calorimetric Determination of the Denaturation Enthalpy for Lysozyme in Sodium Dodecyl Sulfate and Dodecyltrimethylammonium Bromide Solutions. J Solution Chem 37, 619–629 (2008). https://doi.org/10.1007/s10953-008-9267-1

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  • DOI: https://doi.org/10.1007/s10953-008-9267-1

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