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Maghemite Nanoparticles for DNA Extraction: Performance and Blocking Temperature

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Abstract

Iron oxide nanoparticles coated with polyethylene glycol were synthesized by coprecipitation for use in the magnetic separation of DNA (deoxyribonucleic acid). The blocking temperature of nanoparticles was studied by the methods of Mössbauer spectroscopy, ferromagnetic resonance, and using magnetometric measurements. The blocking temperature calculated from the temperature dependence of the coercive force was ~ 200 K. The calculation of the blocking temperature from the relaxation time obtained using Mössbauer spectroscopy gave a value of ~ 450 K. The blocking temperature obtained using ferromagnetic resonance was ~ 910 K. The relationship between the obtained blocking temperatures is in good agreement with the Néel-Brown formula. The constants of effective and surface anisotropy were determined by the method of ferromagnetic resonance. Isolation of DNA from blood using prepared particles and separation in a permanent magnet field revealed sufficient productivity, high speed, and the “chemical delicacy” of this approach.

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Funding

This work was supported by Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support Fund, with research projects no. 20–42-242902. We are grateful to the Center of Collective Use of FRC KSC SB RAS for the provided equipment.

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Authors and Affiliations

  1. Krasnoyarsk Scientific Center, Federal Research Center KSC SB RAS, Krasnoyarsk, Russia

    S. V. Stolyar, A. S. Gorbenko, R. N. Yaroslavtsev, I. A. Olkhovskiy & A. V. Tyumentseva

  2. Kirensky Institute of Physics, Federal Research Center KSC SB RAS, Krasnoyarsk, Russia

    S. V. Stolyar, S. V. Komogortsev, Yu. V. Knyazev, R. N. Yaroslavtsev, O. A. Bayukov & R. S. Iskhakov

  3. Siberian Federal University, Krasnoyarsk, Russia

    S. V. Stolyar

  4. Ural Federal University, Ekaterinburg, Russia

    D. S. Neznakhin

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  1. S. V. Stolyar
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Correspondence to R. N. Yaroslavtsev.

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Stolyar, S.V., Komogortsev, S.V., Gorbenko, A.S. et al. Maghemite Nanoparticles for DNA Extraction: Performance and Blocking Temperature. J Supercond Nov Magn 35, 1929–1936 (2022). https://doi.org/10.1007/s10948-022-06233-5

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