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One pot method to synthesize three-dimensional porous hydroxyapatite nanocomposite for bone tissue engineering

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Abstract

A three-dimensional porous hydroxyapatite nanocomposite has been synthesized by a simple, less energy consuming and cost effective one-pot method. In this study, gelatin foam has been used as pore forming agent and incorporated in carboxymethyl cellulose-hydroxyapatite system in composite formation stage. A three-dimensional porous polymers-hydroxyapatite nanocomposite has been formed as a final product. The synthesized porous nanocomposite has been thoroughly characterized by different techniques. It was found that the nanocomposite is highly porous with almost 80% porosity, and has multi-scale pores from 2.5 to 900 μm in size. Furthermore, the synthesized porous composite has compressive strength ~ 11.8 ± 1.5 MPa and modulus ~ 0.243 ± 0.031 GPa, in the range of cancellous bone. Moreover, the nanocomposite provides favorable environment to cells for proliferation, high alkaline phosphatase (ALP) activity and extracellular mineralization. In vitro degradation of synthesized nanocomposites was tested in simulated body fluid. Results ascertained that the synthesized porous hydroxyapatite nanocomposite would be a promising scaffold for bone tissue engineering.

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Acknowledgements

We would like to thank CSIR-National Metallurgical Laboratory for financial support (OLP-0231) and University Grants Commission for giving fellowship to Chandrani Sarkar.

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Authors and Affiliations

  1. Advanced Materials and Processes Division, CSIR-National Metallurgical Laboratory, Jamshedpur, Jharkhand, 831007, India

    Chandrani Sarkar & Subhadra Garai

  2. Department of Applied Chemistry, Indian Institute of Technology (Indian School of Mines), Dhanbad, Jharkhand, 826004, India

    Chandrani Sarkar & Sumanta Kumar Sahu

  3. Department of Chemistry, Mahila College, Kolhan University, Chaibasa, Jharkhand, 833201, India

    Chandrani Sarkar

  4. Centre for Nanotechnology, Central University of Jharkhand, Ranchi, Jharkhand, 835205, India

    Kumar Anuvrat

  5. CSIR-Central Glass & Ceramic Research Institute, 196, Raja S.C. Mullick Road, Jadavpur, Kolkata, 700 032, India

    Jui Chakraborty

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  1. Chandrani Sarkar
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  2. Kumar Anuvrat
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  3. Subhadra Garai
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Correspondence to Chandrani Sarkar.

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Sarkar, C., Anuvrat, K., Garai, S. et al. One pot method to synthesize three-dimensional porous hydroxyapatite nanocomposite for bone tissue engineering. J Porous Mater 27, 225–235 (2020). https://doi.org/10.1007/s10934-019-00805-y

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