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Fabrication of macroporous chitosan/poly(l-lactide) hybrid scaffolds by sodium acetate particulate-leaching method

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Abstract

For tissue engineering applications, 3-D macroporous chitosan/poly(l-lactide) (PLLA) scaffolds were prepared by the particulate-leaching method using sodium acetate as the porogen in an acidic water/dioxane solution. The stability and dispersity of chitosan on the chitosan/PLLA hybrid scaffolds were determined by measuring water contact angles, establishing crystallinity using X-ray diffraction, and using eosin staining to observe the chitosan under a light microscope. The porous structure of the particulate-leached chitosan/PLLA scaffolds was investigated in terms of pore morphology, interconnectivity, etc. by using scanning electron microscopy. Chitosan/PLLA scaffolds produced by particulate-leaching showed a highly porous structure and improved stability and dispersity of chitosan as compared to pure PLLA and chitosan-coated PLLA scaffolds. The highly porous structure that resulted from a high concentration of chitosan improved the efficiency of cell adhesion after culturing cells for 4 h. After 48 h, the cultured cells showed increased cell proliferation on the hybrid scaffolds. Thus, particulate-leached chitosan/PLLA scaffolds can be applied to tissue engineering of various types, including the industrial membrane field.

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Acknowledgments

This study was supported by a grant from the Health and Medical Technology Research and Development Project of the Ministry for Health, Welfare and Family Affair (#A084152).

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Authors and Affiliations

  1. Department of Biomedical Engineering and Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, Dongdaemun-gu, Seoul, 130-701, Republic of Korea

    Jin Ik Lim & Hun-Kuk Park

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  1. Jin Ik Lim
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  2. Hun-Kuk Park
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Correspondence to Hun-Kuk Park.

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Lim, J.I., Park, HK. Fabrication of macroporous chitosan/poly(l-lactide) hybrid scaffolds by sodium acetate particulate-leaching method. J Porous Mater 19, 383–387 (2012). https://doi.org/10.1007/s10934-011-9485-6

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