Skip to main content
SpringerLink
Log in

Improved catalytic hydrolysis of carboxy methyl cellulose using cellulase immobilized on functionalized meso cellular foam

  • Published:
Journal of Porous Materials Aims and scope Submit manuscript

Abstract

Cellulase from Penicillium funiculosum was immobilized on functionalized MCF (Meso Cellular Foam) silica by imine bond formation followed by reduction using NaBH4. The specific activities of free and immobilized enzyme were measured for hydrolysis of soluble carboxymethyl cellulose (CMC). The highest activity of MCF immobilized and native enzyme was obtained at optimum pH 5 and 4.5 respectively. Kinetic parameters, Michaelis–Menten constant (Km) and maximum reaction velocity (Vmax), were calculated as Km = 0.025 × 10−2 mg/mL, Vmax = 5.327 × 10−3 U/mg for the free enzyme and Km = 0.024 × 10−2 mg/mL, Vmax = 9.794 × 10−3 U/mg for MCF immobilized enzyme respectively. The reusability of immobilized enzymes showed that 66% of its activity is retained even after 15 cycles. The availability of polar groups (–NH–, –OH) and large pore size of surface modified MCF could be electrostatically stabilizing the cellulase. Functionalized MCF was found to be a promising material for stabilizing cellulase with 16.4 wt% loading of enzyme.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Scheme 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11

Similar content being viewed by others

References

  1. B. Hahn-Hägerdal, M. Galbe, M.F. Gorwa-Grauslund, G. Lidén, G. Zacchi, Trends Biotechnol. 24, 549–556 (2006)

    Article  Google Scholar 

  2. P. Sassner, M. Galbe, G. Zacchi, Biomass Bioenergy 32, 422–430 (2008)

    Article  CAS  Google Scholar 

  3. S.J.B. Duff, W.D. Murray, Bioresour. Technol. 55, 1–33 (1996)

    Article  CAS  Google Scholar 

  4. D.J. Gregg, A. Boussaid, J.N. Saddler, Bioresour. Technol. 63, 7–12 (1998)

    Article  CAS  Google Scholar 

  5. M.V. Sivers, G. Zacchi, Bioresour. Technol. 56, 131–140 (1996)

    Article  Google Scholar 

  6. A. Wingren, M. Galbe, G. Zacchi, Biotechnol. Prog. 19, 1109–1117 (2003)

    Article  CAS  Google Scholar 

  7. M. Galbe, G. Zacchi, Appl. Microbio. Biotechnol. 59, 618–628 (2002)

    Article  CAS  Google Scholar 

  8. R. Eklund, M. Galbe, G. Zacchi, Bioresour. Technol. 52, 225–229 (1995)

    Article  CAS  Google Scholar 

  9. W.G. Glasser, R.S. Wright, Biomass Bioenergy 14, 219–235 (1998)

    Article  CAS  Google Scholar 

  10. Y. Sun, J. Cheng, Bioresour. Technol. 83, 1–11 (2002)

    Article  CAS  Google Scholar 

  11. J. Soderstrom, L. Pilcher, M. Galbe, G. Zacchi, Appl. Biochem. Biotechnol. 98, 5–21 (2002)

    Article  Google Scholar 

  12. H.H.P. Yiu, P.A. Wright, J. Mater. Chem. 15, 3690–3700 (2005)

    Article  CAS  Google Scholar 

  13. P.H. Pandya, R.V. Jasra, B.L. Newalker, P.N. Bhatt, Micropor. Mesopor. Mater. 77, 67–77 (2005)

    Article  CAS  Google Scholar 

  14. K. Kannan, R.V. Jasra, J. Mol. Catal. B 56, 34–40 (2009)

    Article  CAS  Google Scholar 

  15. C. Mateo, J.M. Palomo, G. Fernandez-Lorente, J.M. Guisan, R. Fernandez-Lafuente, Enzyme Microb. Technol. 40, 1451–1463 (2007)

    Article  CAS  Google Scholar 

  16. Y.J. Han, T. Watson, G.D. Stucky, A. Butler, J. Mol. Catal. B 17, 1–8 (2002)

    Article  CAS  Google Scholar 

  17. A.B. Jarzębski, K. Szymańska, J. Bryjak, J. Mrowiec-Białoń, Catal. Today 124, 2–10 (2007)

    Article  Google Scholar 

  18. M. Hartmann, Chem. Mater. 17, 4577–4593 (2005)

    Article  CAS  Google Scholar 

  19. D. Jung, C. Streb, M. Hartmann, Int. J. Mol. Sci. 11, 762–778 (2010)

    Article  CAS  Google Scholar 

  20. M. Hartmann, C. Streb, J. Porous. Mater. 13, 347–352 (2006)

    Article  CAS  Google Scholar 

  21. A. Vinu, V. Murugesan, O. Tangermann, M. Hartmann, Chem. Mater. 16, 3056–3065 (2004)

    Article  CAS  Google Scholar 

  22. J. Deere, R.F. De Oliveira, B. Tomaszewski, S. Millar, A. Lalaouni, L.F. Solares, S.L. Flitsch, P.J. Halling, Langmuir 24, 11762–11769 (2008)

    Article  CAS  Google Scholar 

  23. J. Deere, E. Magner, J.G. Wall, B.K. Hodnett, Catal. Lett. 88, 183–186 (2003)

    Article  Google Scholar 

  24. A. Vinu, M. Hartmann, Stud. Surf. Sci. Catal. 154, 2987–2994 (2004)

    Article  Google Scholar 

  25. http://www.rcsb.org/pdb/explore/explore.do?structureId=2BOE

  26. X. Yuan, N. Shan, J. Sheng, X. Wei, J. Membr. Sci. 155, 101–106 (1999)

    Article  CAS  Google Scholar 

  27. B. Afsahi, A. Kazemi, A. Kheirolomoom, S. Nejati, Scientia Iranica 14, 379–383 (2007)

    CAS  Google Scholar 

  28. L. Wu, X. Yuan, J. Sheng, J. Membr. Sci. 250, 167–173 (2005)

    Article  CAS  Google Scholar 

  29. A. Dinçer, A. Telefoncu, J. Mol. Catal. B 45, 10–14 (2007)

    Article  Google Scholar 

  30. M. Paljevac, M. Primožič, M. Habulin, Z. Novak, Z. Knez, J. Supercrit. Fluids. 43, 74–80 (2007)

    Article  CAS  Google Scholar 

  31. A.A.S. Sinegani, G. Emtiazi, H. Shariatmadari, J. Colloid Interface Sci. 290, 39–44 (2005)

    Article  CAS  Google Scholar 

  32. J.S. Lettow, Y.J. Han, P. Schmidt-Winkel, P. Yang, D. Zhao, G.D. Stucky, J.Y. Ying, Langmuir 16, 8291–8295 (2000)

    Article  CAS  Google Scholar 

  33. M.M. Bradford, Anal. Biochem. 72, 248–254 (1976)

    Article  CAS  Google Scholar 

  34. T.K. Ghose, Pure Appl. Chem. 59, 257–268 (1987)

    Article  CAS  Google Scholar 

  35. A.S.M. Chong, X.S. Zhao, J. Phys. Chem. B 107, 12650–12657 (2003)

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Authors are thankful to Director of CSMCRI, for providing access to the instrumentation facility. We thank Mr. Surendra Bade, Regional Sales Manager, Genencor International Asia Pacific Pvt. for providing enzymes. Financial support was provided by the CSIR-SRF(09/955(0002)-2009-EMR-I and Network Project on Catalysis.

Author information

Author notes

  1. Kayambu Kannan & Raksh Vir Jasra

    Present address: R&D Centre Reliance Technology Group, Reliance Industries Limited, Vadodara Manufacturing Division, Vadodara, 391346, Gujarat, India

Authors and Affiliations

  1. Discipline of Inorganic Materials & Catalysis, Central Salt & Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), G.B. Marg, Bhavnagar, Gujarat, 364002, India

    Kayambu Kannan & Raksh Vir Jasra

Authors
  1. Kayambu Kannan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Raksh Vir Jasra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Raksh Vir Jasra.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kannan, K., Jasra, R.V. Improved catalytic hydrolysis of carboxy methyl cellulose using cellulase immobilized on functionalized meso cellular foam. J Porous Mater 18, 409–416 (2011). https://doi.org/10.1007/s10934-010-9392-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10934-010-9392-2

Keywords

Search

Navigation