Abstract
The permeability of metallic and ceramic open-cell foams prepared by the gelcasting technique was assessed by fitting of Forchheimer’s equation to experimental pressure drop curves. The ceramic composition was based on pure hydroxyapatite, while the metallic composition was based on titanium metal. Experimental Darcian (k 1) and non-Darcian (k 2) permeability constants displayed values in the range 0.40–3.24 × 10−9 m2 and 3.11–175.8 × 10−6 m respectively. Tortuosity was evaluated by gas diffusion experiments and ranged from 1.67 to 3.60, with porosity between 72 and 81% and average hydraulic pore size between 325 and 473 μm. Such features were compared to data reported in the literature for cancellous bones and synthetic scaffolds for bone graft. A detailed discussion concerning the limitations of Darcy’s law for fitting laboratory data and for predicting fluid flow through scaffolds in real biomedical applications is also performed. Pore size was obtained by image analysis and was also derived from permeation-absorption-diffusion experiments. In both cases, values were within the range expected for porous scaffolds applications.
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Abbreviations
- A flow :
-
Face area of the sample exposed to flow (m2)
- c :
-
Molar concentration of the gas mixture (mol/m3)
- D AB :
-
Gas diffusivity in air (m2/s)
- D eff :
-
Effective gas diffusivity through the scaffold (m2/s)
- d :
-
Distance between the gas–liquid interface and the bottom face of the sample (m)
- d h :
-
Hydraulic pore diameter (m)
- d sphere :
-
Three-dimensional cell diameter (m)
- d transp :
-
Pore diameter based on transport properties (m)
- Fo :
-
Forchheimer number (−)
- k 1 :
-
Darcian permeability constant (m2)
- k 2 :
-
Non-Darcian permeability constant (m)
- L :
-
Sample thickness along the macroscopic flow direction (m)
- MM A :
-
Molar mass of diffusion gas (mol/kg)
- N AZ :
-
Molar flux of diffusion gas along z-direction (mol/m2.s)
- P :
-
Absolute fluid pressure at which v s, μ and ρ are measured or calculated (Pa)
- P atm :
-
Atmospheric pressure at laboratory location (Pa)
- P i :
-
Absolute fluid pressure at the sample entrance (Pa)
- P o :
-
Absolute fluid pressure at the sample exit (Pa)
- P VA :
-
Absolute vapor pressure of diffusion gas (Pa)
- Q :
-
Volumetric flow rate (m3/s)
- R :
-
Ideal gas constant (Pa m3/mol K)
- Re pore :
-
Reynolds number at the pore level (−)
- S :
-
Cross-sectional sample surface exposed to vapor diffusion (m2)
- T :
-
Temperature of the fluid (K)
- v i :
-
Interstitial fluid velocity (m/s)
- v s :
-
Face or superficial fluid velocity (m/s)
- z :
-
Distance in diffusion direction (m)
- y :
-
Molar fraction of gas mixture (−)
- Δm :
-
Mass variation measured during the diffusion experiment (kg)
- ΔP :
-
Pressure drop through the medium (Pa)
- ΔP viscous :
-
Pressure drop due to viscous effects (Pa)
- ΔP inertial :
-
Pressure drop due to inertial effects (Pa)
- Δt :
-
Duration of the diffusion experiment (s)
- ε :
-
Porosity of the medium (−)
- μ :
-
Absolute fluid viscosity (Pa s)
- ρ :
-
Fluid density (kg/m3)
- ρ b :
-
Bulk density of the scaffold (kg/m3)
- ρ s :
-
Density of the solid fraction (kg/m3)
- τ :
-
Tortuosity of the scaffold (−)
References
P.K. Chu, X. Liu (eds.), Biomaterials Fabrication and Processing Handbook (CRC Press, Boca Raton, 2008)
M.M. Stevens, Biomaterials for bone tissue engineering. Mater Today 11(5), 18–25 (2008). doi:10.1016/S1369-7021(08)70086-5
S. Oh, N. Oh, M. Appleford, J.L. Ong, Bioceramics for tissue engineering applications–A review. Am. J. Biochem. Biotechnol. 2(2), 49–56 (2006)
M. Scheffler, P. Colombo (eds.), Cellular Ceramics: Structure, Manufacturing, Properties and Applications (Wiley, New York, 2005)
H. Nakajima, Fabrication, properties and application of porous metals with directional pores. Prog. Mater. Sci. 52, 1091–1173 (2007). doi:10.1016/j.pmatsci.2006.09.001
R. Singh, P.D. Lee, T.C. Lindley, R.J. Dashwood, E. Ferrie, T. Imwinkelried, Characterization of the structure and permeability of titanium foams for spinal fusion devices. Acta Biomater. 5, 477–487 (2009). doi:10.1016/j.actbio.2008.06.014
L.M.R. Vasconcellos, M.V. Oliveira, M.L.A. Graça, L.G.O. Vasconcellos, Y.R. Carvalho, C.A.A. Cairo, Porous titanium scaffolds produced by powder metallurgy for biomedical applications. Mater Res. 11(3), 275–280 (2008). doi:10.1590/S1516-14392008000300008
S.C.P. Cachinho, R.N. Correia, Titanium scaffolds for osteointegration: mechanical, in vitro and corrosion behaviour. J. Mater Sci. Mater Med. 19(1), 451–457 (2008). doi:10.1007/s10856-006-0052-7
E. Zhang, C. Zou, Porous titanium and silicon-substituted hydroxyapatite biomodification prepared by a biomimetic process: characterization and in vivo evaluation. Acta Biomater. (2009). doi: 10.1016/j.actbio.2009.01.014
H. Petite, R. Quarto (eds.), Engineered Bone (Tissue Engineering Intelligence Unit) (Landes Bioscience, Austin, 2005)
M.D.M. Innocentini, P. Sepulveda, V.R. Salvini, J.R. Coury, V.C. Pandolfelli, Permeability and structure of cellular ceramics: a comparison between two preparation techniques. J. Am. Ceram. Soc. 81(12), 3349–3352 (1998). doi:10.1111/j.1151-2916.1998.tb02782.x
S. Li, J.R. De Wijn, J. Li, P. Layrolle, K. De Groot, Macroporous biphasic calcium phosphate scaffold with high permeability/porosity ratio. Tissue Eng. 9(3), 535–548 (2003). doi:10.1089/107632703322066714
P. Sepulveda, F. Ortega, M.D.M. Innocentini, V.C. Pandolfelli, Properties of highly porous hydroxyapatite obtained by the gelcasting of foams. J. Am. Ceram. Soc. 83(12), 3021–3024 (2001)
S. Impens, R. Schelstraete, J. Luyten, S. Mullens, I. Thijs, J. Van Humbeeck, J. Schrooten, Production and characterization of porous calcium phosphate structures with controllable hydroxyapatite/b-tricalcium phosphate ratios. Proceedings of the 10th international conference on ceramic processing science, May 25–28, 2008 in Inuyama, Japan
C.M. Agrawal, J.S. McKinney, D. Lanctot, K.A. Athanasiou, Effects of fluid flow on the in vitro degradation kinetics of biodegradable scaffolds for tissue engineering. Biomaterials 21, 2443–2452 (2000). doi:10.1016/S0142-9612(00)00112-5
M.D.M. Innocentini, V.R. Salvini, A. Macedo, V.C. Pandolfelli, Prediction of ceramic foams permeability using Ergun’s equation. Mater Res. 2(4), 283–289 (1999). doi:10.1590/S1516-14391999000400008
M.D.M. Innocentini, P. Sepulveda, F. Ortega, Permeability, in Cellular Ceramics: Structure, Manufacturing, Properties and Applications, ed. by M. Scheffler, P. Colombo (Wiley, New York, 2005), pp. 313–340
A.A. Garrouch, L. Ali, F. Qasem, Using diffusion and electrical measurements to assess tortuosity of porous media. Ind. Eng. Chem. Res. 40, 4363–4369 (2001). doi:10.1021/ie010070u
B. Starly, E. Yildirim, W. Sun, A tracer metric numerical model for predicting tortuosity factors in three-dimensional porous tissue scaffolds. Comput. Methods Programs Biomed. 87, 21–27 (2007). doi:10.1016/j.cmpb.2007.04.003
L. Shen, Z. Chen, Critical review of the impact of tortuosity on diffusion. Chem. Eng. Sci. 62, 3748–3755 (2007). doi:10.1016/j.ces.2007.03.041
R.B. Bird, W.E. Steward, Lightfoot EN Transport Phenomena (Wiley, New York, 1960)
J.R. Welty, C.E. Wicks, R.E. Wilson, Fundamentals of Momentum, Heat and Mass Transfer (Wiley, New York, 1984)
R.M. Felder, R.W. Rousseau, Elementary Principles of Chemical Processes, 3rd edn. (Wiley, New York, 1999)
M.D.M. Innocentini, V.R. Salvini, J.R. Coury, V.C. Pandolfelli, The Permeability of ceramic foams. Am Ceram Soc Bull 78(9), 78–84 (1999)
E.A. Moreira, M.D.M. Innocentini, J.R. Coury, Permeability of ceramic foams to compressible and incompressible flow. J. Eur. Ceram Soc. 24(10–11), 3209–3218 (2004). doi:10.1016/j.jeurceramsoc.2003.11.014
L. Biasetto, P. Colombo, M.D.M. Innocentini, S. Mullens, Gas permeability of microcellular ceramic foams. Ind. Eng. Chem. Res. 46, 3366–3372 (2007). doi:10.1021/ie061335d
D. Seguin, A. Montillet, J. Comiti, Experimental characterisation of flow regimes in various porous media–I: Limit of laminar flow regime. Chem. Eng. Sci. 53(21), 3751–3761 (1998). doi:10.1016/S0009-2509(98)00175-4
D. Seguin, A. Montillet, J. Comiti, F. Huet, Experimental characterisation of flow regimes in various porous media–II: Transition to turbulent regime. Chem. Eng. Sci. 53(22), 3897–3909 (1998). doi:10.1016/S0009-2509(98)80003-1
D. Hlushkou, U. Tallarek, Transition from creeping via viscous-inertial to turbulent flow in fixed beds. J. Chromatogr. A 1126, 70–85 (2006). doi:10.1016/j.chroma.2006.06.011
S. Mullens, J. Luyten, J. Zeschky, Characterization of Structure and Morphology, in Cellular Ceramics: Structure, Manufacturing, Properties and Applications, ed. by M. Scheffler, P. Colombo (Wiley, New York, 2005), pp. 227–266
I. Ochoa, J.A. Sanz-Herrera, J.M. Garcia-Aznar, M. Doblaré, D.M. Yunos, A.R. Boccaccini (2008) Permeability evaluation of 45S5 Bioglass-based scaffolds for bone tissue engineering. J Biomech. doi:10.1016/j-jbiomech.2008.10.030
A.C. Jones, C.H. Arns, D.W. Hutmacher, B.K. Milthorpe, A.P. Sheppard, M.A. Knackstedt, The correlation of pore morphology, interconnectivity and physical properties of 3D ceramic scaffolds with bone ingrowth. Biomaterials 30, 1440–1451 (2009). doi:10.1016/j.biomaterials.2008.10.056
V. Karageorgiou, D. Kaplan, Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 26, 5474–5491 (2005). doi:10.1016/j.biomaterials.2005.02.002
W. Lauriks, J. Thoen, I.V. Asbroeck, G. Lowet, G.V.D. Perre, Propagation of ultrasonic pulses through trabecular bone, Journal de Physique IV, colloque C5, supplement au Journal de Physique III, vol. 4, may 1994
J.A. Sanz-Herrera, J.M. García-aznar, M. Doblaré, On scaffold designing for bone regeneration: A multiscale approach. Acta Biomater. 5, 219–229 (2009). doi:10.1016/j.actbio.2008.06.021
P.W. Hui, P.C. Leung, A. Sher, Fluid conductance of cancellous bone graft as a predictor for graft-host interface healing. J. Biomech. 29(1), 123–132 (1996). doi:10.1016/0021-9290(95)00010-0
A.J. Beaudoin, W.M. Mihalko, W.R. Krause, Finite element modeling of polymethylmethacrylate flow through cancellous bone. J. Biomech. 24(2), 127–136 (1991). doi:10.1016/0021-9290(91)90357-S
S.S. Kohles, J.B. Roberts, M.L. Upton, C.G. Wilson, L.J. Bonassar, A.L. Schlichting, Direct perfusion measurements of cancellous bone anisotropic permeability. J. Biomech. 34, 1197–1202 (2001). doi:10.1016/S0021-9290(01)00082-3
T.H. Lim, J.H. Hong, Poroelastic properties of bovine vertebral trabecular bone. J. Orthop. Res. 18(4), 671–677 (2000). doi:10.1002/jor.1100180421
E.A. Nauman, K.E. Fong, T.M. Keaveny, Dependence of intertrabecular permeability on flow direction and anatomic site. Ann. Biomed. Eng. 27, 517–524 (1999). doi:10.1114/1.195
J.A. Ochoa, B.M. Hillberry (1992) Permeability of bovine cancellous bone. Transactions ORS17:162
M.J. Grimm, J.L. Williams, Measurement of permeability in calcaneal trabecular bone. J. Biomech. 30(7), 743–745 (1997). doi:10.1016/S0021-9290(97)00016-X
D.A. Shimko, V.F. Shimko, E.A. Sander, K.F. Dickson, E.A. Nauman, Effect of porosity on the fluid flow characteristics and mechanical properties of tantalum scaffolds. J. Biomed. Mater. Res. B 73, 315–324 (2005)
S.M. Haddock, J.C. Debes, E.A. Nauman, K.E. Fong, Y.P. Arramon, T.M. Keaveny, Structure–function relationships for coralline hydroxyapatite bone substitute. J. Biomed. Mater. Res. 47, 71–78 (1999). doi:10.1002/(SICI)1097-4636(199910)47:1<71:AID-JBM10>3.0.CO;2-U
P. Swider, M. Conroy, A. Pédrono, D. Ambard, S. Mantell, K. Soballe, J.E. Bechtold, Use of high-resolution MRI for investigation of fluid flow and global permeability in a material with interconnected porosity. J Biomech 40, 2112–2118 (2007). doi:10.1016/j.jbiomech.2006.10.002
F.J. O’Brien, B.A. Harley, M.A. Waller, I.V. Yannas, L.J. Gibson, P.J. Prendergast, The effect of pore size on permeability and cell attachment in collagen scaffolds for tissue engineering. Technol. Health Care 15, 3–17 (2007)
D. Ruth, H. Ma, On the derivation of the Forchheimer equation by means of the averaging theorem. Transp. Porous Media 7, 255–264 (1992). doi:10.1007/BF01063962
R.H. Vera, E. Genové, L. Alvarez, S. Borrós, R. Kamm, D. Lauffenburger, C.E. Semino, Interstitial fluid flow intensity modulates endothelial sprouting in restricted Src-activated cell clusters during capillary morphogenesis. Tissue Eng Part A 15(1), 175–185 (2009). doi:10.1089/ten.tea.2007.0314
C. Sandino, J.A. Planell, D. Lacroix, A finite element study of mechanical stimuli in scaffolds for bone tissue engineering. J. Biomech. 41, 1005–1014 (2008). doi:10.1016/j.jbiomech.2007.12.011
R.M. Dillaman, R.D. Roer, D.M. Gay, Fluid movement in bone: theoretical and empirical. J. Biomech. 24(Suppl 1), 163–177 (1991). doi:10.1016/0021-9290(91)90386-2
R.J. Montgomery, B.D. Sutker, J.T. Bronk, S.R. Smith, P.J. Kelly, Interstitial fluid flow in cortical bone. Microvasc. Res. 35, 295–307 (1988). doi:10.1016/0026-2862(88)90084-2
I.D. McCarthy, L. Yang, A distributed model of exchange processes within the osteon. J. Biomech. 25, 441–450 (1992). doi:10.1016/0021-9290(92)90263-Z
M.V. Hillsley, J.A. Frangos, Review: Bone tissue engineering: The role of interstitial fluid flow. Biotechnol. Bioeng. 43, 573–581 (1994). doi:10.1002/bit.260430706
P. Moldrup, T. Olesen, T. Komatsu, P. Schjønning, D.E. Rolston, Tortuosity, diffusivity, and permeability in the soil liquid and gaseous phases. Soil Sci. Soc. Am. J. 65, 613–623 (2001)
R.J. Millington, J.M. Quirk, Formation factor and permeability equations. Nature 202, 143–145 (1964). doi:10.1038/202143a0
B.C. Ball, M.F. O’Sullivan, R. Hunter, Gas diffusion, fluid flow and derived pore continuity indices in relation to vehicle traffic and tillage. J. Soil Sci. 39, 327–339 (1988). doi:10.1111/j.1365-2389.1988.tb01219.x
Acknowledgments
The authors would like to thank VITO for supplying samples and MCT/CNPq, Process 471814/2088 3, for the financial support to this work.
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Innocentini, M.D.M., Faleiros, R.K., Pisani, R. et al. Permeability of porous gelcast scaffolds for bone tissue engineering. J Porous Mater 17, 615–627 (2010). https://doi.org/10.1007/s10934-009-9331-2
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DOI: https://doi.org/10.1007/s10934-009-9331-2