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Expression, Purification and Characterization of Recombinant Disintegrin from Gloydius Brevicaudus Venom in Escherichia Coli

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Abstract

Disintegrins, a family of snake venom protein, which are capable of modulating the activity of integrins that play a fundamental role in the regulation of many physiological and pathological processes. The main purpose of this study is to obtain the recombinant disintegrin (r-DI) and evaluate its biological activity. In this study, we explored a high-level expression prokaryotic system and purification strategy for r-DI. Then, r-DI was treated to assay effects on cell growth, migration, and invasion. The affinity for the interactions of r-DI with integrin was determined using Surface plasmon resonance (SPR) analyses. The r-DI can be expressed in Escherichia coli and purified by one-step chromatography. The r-DI can inhibit B16F10 cells proliferation, migration, and invasion. Also, we found that r-DI could interact with the integrin αIIbβ3 (GPIIb/IIIa). The r-DI can be expressed, purified, characterized through functional assays, and can also maintain strong biological activities. Thus, this study showed potential therapeutic effects of r-DI for further functional and structural studies.

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Data Availability

No datasets were generated or analysed during the current study.

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Funding

This work was supported by Startup Fund for scientific research, Fujian Medical University(Grant number: 2022QH1216).

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Authors and Affiliations

  1. Department of Pharmacy, Fujian Children’s Hospital (Fujian Branch of Shanghai Children’s Medical Center), College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, FuJian Medical University, Fuzhou, Fujian, China

    Yinxiang Lan

  2. Department of Pharmacy, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, Fujian, China

    Xiuliang Qiu

  3. The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian, China

    Yunlu Xu

  4. Center of Translational Hematology of Fujian Medical University, Fuzhou, Fujian, China

    Yunlu Xu

Authors
  1. Yinxiang Lan
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  2. Xiuliang Qiu
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  3. Yunlu Xu
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Contributions

Yinxiang Lan and Xiuliang Qiu wrote the main manuscript text， Yinxiang Lan and Xiuliang Qiu prepared figures. All authors reviewed the manuscript.

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Correspondence to Yunlu Xu.

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Lan, Y., Qiu, X. & Xu, Y. Expression, Purification and Characterization of Recombinant Disintegrin from Gloydius Brevicaudus Venom in Escherichia Coli. Protein J (2024). https://doi.org/10.1007/s10930-024-10198-w

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  • DOI: https://doi.org/10.1007/s10930-024-10198-w

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