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Determining the Protein Stability of Alzheimer’s Disease Protein, Amyloid Precursor Protein

  • Alexandré Delport
  • Raymond HewerEmail author
Article
  • 51 Downloads

Abstract

Determining protein thermal stability is integral in biomedical research. Here, with the use of two thermal stability assays, we show the melting temperature of amyloid precursor protein, an Alzheimer’s disease related protein. The average melting temperature for amyloid precursor protein of 55.9 °C was derived from differential scanning fluorometry (55.1 ± 0.3 °C) and cellular thermal melt (56.7 ± 0.7 °C). These experimental methods have significant application for Alzheimer’s disease research including their use for amyloid precursor protein stability profiling and for the identification of additional binding partners to further elucidate novel protein functions.

Keywords

Differential scanning fluorometry Cellular thermal shift assay Amyloid precursor protein Alzheimer’s disease 

Abbreviations

TSA

Thermal shift assay

CETSA

Cellular thermal shift assay

DSF

Differential scanning fluorometry

AD

Alzheimer’s disease

APP

Amyloid precursor protein

IMAC

Immobilized metal-ion affinity chromatography

Notes

Acknowledgements

We would like to acknowledge the National Research Foundation/Deutscher Akademischer Austauschdienst (DAAD170613239977) and the University of KwaZulu-Natal for funding and permission to publish the research produced here. The authors declare that there are no conflicts of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Discipline of Biochemistry, School of Life SciencesUniversity of KwaZulu-NatalPietermaritzburgSouth Africa

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