The Protein Journal

, Volume 37, Issue 1, pp 21–37 | Cite as

Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome

  • Ivan Mikšík
  • Statis Paradis
  • Adam Eckhardt
  • David Sedmera


The proteins and pigment of the eggshell of the Siamese crocodile (Crocodylus siamensis) were analysed. For proteomic analysis, various decalcification methods were used when the two main surface layers were analyzed. These layers are important for antimicrobial defense of egg (particularly the cuticle). We found 58 proteins in both layers, of which 4 were specific for the cuticle and 26 for the palisade (honeycomb) layer. Substantial differences between proteins in the eggshell of crocodile and previously described birds’ eggshells exist (both in terms of quality and quantity), however, the entire proteome of Crocodilians has not been described yet. The most abundant protein was thyroglobulin. The role of determined proteins in the eggshell of the Siamese crocodile is discussed. For the first time, the presence of porphyrin pigment is reported in a crocodilian eggshell, albeit in a small amount (about 2 to 3 orders of magnitude lower than white avian eggs).


Proteins of eggshell Eggshell pigment Crocodile 



We greatly appreciate the donation of crocodile’s eggshells by Ing. Procházka from the Crocodile ZOO Protivín. Financial support from the Czech Science Foundation (GA CR) (15-01948S - IM, SP, AE and 16-02972S - DS) is gratefully acknowledged.

Compliance with Ethical Standards

Conflict of interest

All authors declare that there are no competing interests.

Supplementary material

10930_2017_9750_MOESM1_ESM.pdf (124 kb)
Supplementary material 1 (PDF 124 KB)


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© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Institute of Physiology of the Czech Academy of SciencesPrague 4Czech Republic
  2. 2.Institute of Anatomy, First Faculty of MedicineCharles UniversityPrague 4Czech Republic

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