Skip to main content

Advertisement

SpringerLink
Log in

Proteomic Analysis of Rice Seedlings Under Cold Stress

  • Published:
The Protein Journal Aims and scope Submit manuscript

Abstract

Low temperature can greatly restrict the growth and development of rice. The rice seedlings show growth retardation, lamina wrap, and part of blade even died under the condition of low temperature. In order to get more information about cold stress responses in rice, two dimensional electrophoresis and bioinformatics analysis of mass spectrometry were used to preliminary survey the cold tolerance of cold sensitive line 9311 and cold resistance variety Fujisaka 5 under cold stress. Two dimensional electrophoresis maps of 9311 and Fujisaka 5 were established under cold treatment. With analysis of bioinformation, the proteins were found involve in many aspects of rice development. The largest category of proteins is functioning on metabolism. By comparing the proteins from the two varieties, it can be found that most proteins from 9311 were down-regulated and were up-regulated in Fujisaka 5. The results showed that the membrane composition and structure were damaged, metabolism changed dramatically and rice defense system was activated under the cold stimulation. Fifty-nine proteins related to the resistance of cold stress were identified in our study, and we have investigated and classified all of their biological functions. The importance of our study are providing some conduct for the research of rice resistant to cold stress, supporting auxiliary technique for rice varieties and widening the search field of cold tolerance in plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

2-DE:

Two dimensional electrophoresis

MS:

Mass spectrometry

iTRAQ:

Isobaric tags for relative and absolute quantitation

Rubisco LSU:

Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit

IPG:

Immobilized pI gradient

IEF:

Isoelectric focusing

MALDI TOF:

Matrix-assisted laser desorption ionization time-of-flight

References

  1. Cui SX, Huang F, Wang J, Ma X, Cheng Y, Liu J (2005) A proteomic analysis of cold stress responses in rice seedlings. Proteomics 5:3162–3172

    Article  CAS  Google Scholar 

  2. Imin N, Kerim T, Rolfe BG, Weinman J (2004) Effect of early cold stress on the maturation of rice anthers. Proteomics 4:1873–1882

    Article  CAS  Google Scholar 

  3. Hayashi T, Yamaguchi T, Nakayama K, Komatsu S, Koike S (2006) Susceptibility to coolness at the young microspore stage under high nitrogen supply in rice (Oryza sativa L.). Proteome analysis of mature anthers. Plant Prod Sci 9:212–218

    Article  CAS  Google Scholar 

  4. Vergnolle C, Vaultier MN, Taconnat L, Renou JP, Kader JC, Zachowski A, Ruelland E (2005) The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions. Plant Physiol 139:1217–1233

    Article  CAS  Google Scholar 

  5. Lee BH, Henderson DA, Zhu JK (2005) The Arabidopsis cold-responsive transcriptome and its regulation by ICE1. Plant Cell 17:3155–3175

    Article  CAS  Google Scholar 

  6. Buchanan CD, Lim S, Salzman RA, Kagiampakis I, Morishige DT, Weers BD, Klein RR, Pratt LH, Cordonnier-Pratt MM, Klein PE, Mullet JE (2005) Sorghum bicolor’s transcriptome response to dehydration, high salinity and ABA. Plant Mol Biol 58:699–720

    Article  CAS  Google Scholar 

  7. Rabbani MA, Maruyama K, Abe H, Khan MA, Katsura K, ItoY, Yoshiwara K, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Monitoring expression profiles of rice genes under cold, drought, and high-salinity stress and abscisic acid application using cDNA microarray and RNA gel-blot analyses. Plant Physiol 133:1755–1767

    Article  CAS  Google Scholar 

  8. Kawamura Y, Uemura M (2003) Mass spectrometric approach for identifying putative plasma membrane proteins of Arabidopsis leaves associated with cold acclimation. Plant J 36:141–154

    Article  CAS  Google Scholar 

  9. Xu J, Li Y, Sun J, Du L, Zhang Y, Yu Q, Liu X (2013) Comparative physiological and proteomic response to abrupt low temperature stress between two winter wheat cultivars differing in low temperature tolerance. Plant Biol 15:292–303

    Article  CAS  Google Scholar 

  10. Yang QS, Wu JH, Li CY, Wei YR, Sheng O, Hu CH, Kuang RB (2012) Quantitative proteomic analysis reveals that antioxidation mechanisms contribute to cold tolerance in plantain (Musa paradisiaca L.; ABB Group) seedlings. Mol Cell Proteom 11:1853–1869

    Article  Google Scholar 

  11. Komatsu S, Karibe H, Hamada T, Rakwal R (1999) Phosphorylation upon cold stress in rice (Oryza sativa L.) seedlings. Theor Appl Genet 9:1304–1310

    Article  Google Scholar 

  12. Tsugita A, Kamo M (1999) 2-D electrophoresis of plant proteins. Methods Mol Biol 112:95–97

    CAS  Google Scholar 

  13. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  14. Wessel D, Flugge UI (1984) A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal Biochem 138:141–143

    Article  CAS  Google Scholar 

  15. Huang F, Parmryd I, Nilsson F, Persson AL, Pakrasi HB, Andersson B, Norling B (2002) Proteomics of Synechocystis sp. strain PCC 6803 identification of plasma membrane proteins. Mol Cell Proteom 1:956–966

    Article  CAS  Google Scholar 

  16. Fulda S, Huang F, Nilsson F, Hagemann M, Norling B (2000) Proteomics of Synechocystis sp. strain PCC 6803 identification of periplasmic proteins in cells grown at low and high salt concentrations. Eur J Biochem 267:5900–5907

    Article  CAS  Google Scholar 

  17. Jin BF, He K, Wang HX, Wang J, Zhou T, Lan Y, Hu MR, Wei KH, Yang SC, Shen BF, Zhang XM (2003) Proteomic analysis of ubiquitin-proteasome effects: insight into the function of eukaryotic initiation factor 5 A. Oncogene 22:4819–4830

    Article  CAS  Google Scholar 

  18. Jie Zou, Cuifang Liu, Xinbo Chen (2011) Proteomics of rice in response to heat stress and advances in genetic engineering for heat tolerance in rice. Plant Cell Rep 30:2155–2165

    Article  CAS  Google Scholar 

  19. Gammulla CG, Pascovici D, Atwell BJ, Haynes PA (2011) Differential proteomic response of rice (Oryza sativa) leaves exposed to high- and low-temperature stress. Proteomics 11:2839–2850

    Article  CAS  Google Scholar 

  20. Yang PF, Li XJ, Liang Y, Jing YX, Shen SH, Kuang TY (2006) Proteomic analysis of the response of Liangyoupeijiu (super high-yield hybrid rice) seedlings to cold stress. J Integr Plant Biol 48(8):945–951

    Article  CAS  Google Scholar 

  21. Ma Y, Dai XY, Xu YY, Luo W, Zheng XM, Zeng D, Pan YJ, Lin XL, Liu HH, Zhang DJ, Xiao J, Guo XY, Xu SJ, Niu YD, Jin JB, Zhang H, Xu X, Li LG, Wang W, Qian Q, Ge S, Chong K (2015) COLD1 confers chilling tolerance in rice. Cell 160(6):1209–1221

    Article  CAS  Google Scholar 

  22. Chen XH, Zhang WF, Zhang BQ, Zhou JC, Wang YF, Yang QB, Ke YQ, He HQ (2011) Phosphoproteins regulated by heat stress in rice leaves. Proteom Sci 9:37–45

    Article  Google Scholar 

  23. Makoto Hashimoto, Setsuko Komatsu (2007) Proteomic analysis of rice seedlings during cold stress. Proteomics 7:1293–1302

    Article  CAS  Google Scholar 

  24. Yan SP, Zhang QY, Tang ZC, Su WA, Sun WN (2006) Comparative proteomic analysis provides new insights into chilling stress responses in rice. Mol Cell Proteom 5:484–496

    Article  CAS  Google Scholar 

  25. Lee DG, Ahsan N, Lee JJ, Bahk JD, Kang KY, Lee BH (2009) Chilling stress-induced proteomic changes in rice roots. J Plant Physiol 166:1–11

    Article  CAS  Google Scholar 

  26. Neilson KA, Mariani M, Haynes PA (2011) Quantitative proteomic analysis of cold-responsive proteins in rice. Proteomics 11:1696–1706

    Article  Google Scholar 

  27. Zhang ZG, Zhang QA, Wu JX, Zheng X, Zheng S, Sun XH, Qiu QS, Lu TG (2013) Gene knockout study reveals that cytosolic ascorbate peroxidase 2 (OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses. PLoS ONE 8(2):e57472

    Article  CAS  Google Scholar 

  28. Fowler S, Thomashow MF (2002) Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell 14:1675–1690

    Article  CAS  Google Scholar 

  29. Chen JH, Tian L, Xu HF, Tian DG, Luo YM, Ren CM, Yang LM, Shi JS (2012) Cold-induced changes of protein and phosphoprotein expression patterns from rice roots as revealed by multiplex proteomic analysis. Plant Omics J 5(2):194–199

    CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the Outstanding Young Teacher Training Project in High Education Institutions of Guangxi Province (No. GXQG022014003).

Author information

Authors and Affiliations

  1. Key Laboratory of Plant Genetics and Breeding, Agricultural College, Guangxi University, Nanning, 530004, China

    Li Ji, Ping Zhou, Ya Zhu, Fang Liu, Rongbai Li & Yongfu Qiu

  2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi University, Nanning, 530004, China

    Fang Liu, Rongbai Li & Yongfu Qiu

Authors
  1. Li Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ping Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ya Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rongbai Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yongfu Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Rongbai Li or Yongfu Qiu.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Human Participants and Animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ji, L., Zhou, P., Zhu, Y. et al. Proteomic Analysis of Rice Seedlings Under Cold Stress. Protein J 36, 299–307 (2017). https://doi.org/10.1007/s10930-017-9721-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10930-017-9721-2

Keywords

Search

Navigation