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Preliminary Crystallographic Analysis of a Cruciferin Protein from Seeds of Moringa oleifera

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Abstract

A 55 kDa cruciferin protein has been purified and characterized from seeds of Moringa oleifera plant. Protein blast of N-terminal amino-acid sequence showed 60 % sequence similarity with cruciferin from Brassica napus. The M. oleifera protein has been crystallized applying the sitting drop method using 5 % polyethylene glycol 8,000, 38.5 % 3-methyl-1,5-pentanediol and 0.1 M sodium cacodylate pH 6.5. The crystals belonged to the P6322 hexagonal space group with cell dimensions, a = b = 98.4, c = 274.3 Å. Initial diffraction data have been collected to a resolution of 6 Å.

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Abbreviations

PEG:

Polyethylene glycol

MPD:

3-Methyl-1,5-pentanediol

MO:

Moringa oleifera

DESY:

Deutsches Elektronen Synchrotron

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

DLS:

Dynamic light scattering

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Acknowledgments

The work was supported by Higher Education Commission (HEC), Pakistan and Deutscher Akademischer Austausch Dienst (DAAD), Germany under the collaborative project “Ph.D scholarships for Engineering & Sciences in Germany’’ as a part of Human Resource Development (HRD) plan of Pakistan government.

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Authors and Affiliations

  1. Laboratory for Structural Biology of Infection and Inflammation, Department of Chemistry, c/o DESY, University of Hamburg, Notkestrasse 85, 22603, Hamburg, Germany

    Ahmed Akrem, Nasser Yousef, Afshan Begum, Arne Meyer, Markus Perbandt & Christian Betzel

  2. Biochemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt

    Amr Negm

  3. Department of Botany, Institute of Pure and Applied Biology, Bahauddin Zakariya University, 60800, Multan, Pakistan

    Ahmed Akrem

  4. Institute for Clinical Chemistry, University Medical Centre Hamburg-Eppendorf, Martinistr. 52, 20246, Hamburg, Germany

    Friedrich Buck

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  1. Ahmed Akrem
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  2. Nasser Yousef
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  3. Afshan Begum
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  4. Amr Negm
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  5. Arne Meyer
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  6. Markus Perbandt
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  7. Friedrich Buck
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  8. Christian Betzel
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Correspondence to Ahmed Akrem.

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Akrem, A., Yousef, N., Begum, A. et al. Preliminary Crystallographic Analysis of a Cruciferin Protein from Seeds of Moringa oleifera . Protein J 33, 253–257 (2014). https://doi.org/10.1007/s10930-014-9558-x

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  • DOI: https://doi.org/10.1007/s10930-014-9558-x

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