Abstract
A novel gene encoding thermostable endoglucanase was identified in Xanthomonas sp. EC102 from soil. The gene had 1,458 base pairs of open reading frame, which encode a 52-kDa protein of 486 amino acid residues. Sequence of the amino acid residues was similar with the endoglucanase from Xanthomonas campestris pv. campestris ATCC33913 (GenBank Accession No. NP_638867.1) (94 % identity). The endoglucanase was overexpressed in Escherichia coli BL21 and purified. Temperature for the highest enzymatic activity was 70 °C and pH optima was pH 5.5. The specific activity of the endoglucanase toward carboxymethylcellulose (CMC) was approximately 2 μmol min−1 mg−1, V max for CMC was 1.44 μmol mg−1 min−1, and K m values was 25.6 mg mL−1. The EC102 endoglucanase was stable at temperatures up to 60 °C, and it was activated by 0.1 mM of Mn2+ and Co2+. This is the first report about thermostable endoglucanase from Xanthomonas sp.
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Abbreviations
- CMC:
-
Carboxymethylcellulose
- GH 5:
-
Glycosyl hydrolase family 5
- CBM2:
-
Carbohydrate-binding module 2
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Acknowledgments
This work was supported by Grants from the KRIBB Initiative Program and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (Grant Number RBM4381313).
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Woo, MH., Chang, YH., Lee, HS. et al. First Thermostable Endo-β-1,4-Glucanase from Newly Isolated Xanthomonas sp. EC102. Protein J 33, 110–117 (2014). https://doi.org/10.1007/s10930-013-9535-9
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DOI: https://doi.org/10.1007/s10930-013-9535-9