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Complete Amino Acid Sequence of Globin Chains and Biological Activity of Fragmented Crocodile Hemoglobin (Crocodylus siamensis)

The Protein Journal volume 31, pages 466–476 (2012)Cite this article

Abstract

Hemoglobin, α-chain, β-chain and fragmented hemoglobin of Crocodylus siamensis demonstrated both antibacterial and antioxidant activities. Antibacterial and antioxidant properties of the hemoglobin did not depend on the heme structure but could result from the compositions of amino acid residues and structures present in their primary structure. Furthermore, thirteen purified active peptides were obtained by RP-HPLC analyses, corresponding to fragments in the α-globin chain and the β-globin chain which are mostly located at the N-terminal and C-terminal parts. These active peptides operate on the bacterial cell membrane. The globin chains of Crocodylus siamensis showed similar amino acids to the sequences of Crocodylus niloticus. The novel amino acid substitutions of α-chain and β-chain are not associated with the heme binding site or the bicarbonate ion binding site, but could be important through their interactions with membranes of bacteria.

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Abbreviations

Hb:

Hemoglobin

cHb:

Crocodylus siamensis hemoglobin

TFA:

Trifluoroacetic acid

ACN:

Acetonitrile

DPPH:

1,1-Diphenyl-2-picrylhydrazyl

PBS:

Phosphate buffer saline

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Acknowledgments

This investigation was supported by Thailand Research Fund, Graduate School of Khon Kaen University, Protein and Proteomics Research Group Khon Kaen University and Rajamangala University of Technology, Isan Surin Campus. We wish to acknowledge the support of the Khon Kaen University Publication Clinic, Research and Technology Transfer Affairs, Khon Kaen University, for their assistance.

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Affiliations

  1. Protein and Proteomics Research Group, Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Saowaluck Srihongthong, Anawat Pakdeesuwan, Sakda Daduang & Sompong Thammasirirak

  2. Department of Bioscience, School of Agriculture, Tokai University, Aso, Kumamoto, 869-140, Japan

    Tomohiro Araki

  3. Faculty of Humanities and Social Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand

    Apisak Dhiravisit

Authors
  1. Saowaluck Srihongthong
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  2. Anawat Pakdeesuwan
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  3. Sakda Daduang
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  4. Tomohiro Araki
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  5. Apisak Dhiravisit
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  6. Sompong Thammasirirak
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Correspondence to Sompong Thammasirirak.

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Srihongthong, S., Pakdeesuwan, A., Daduang, S. et al. Complete Amino Acid Sequence of Globin Chains and Biological Activity of Fragmented Crocodile Hemoglobin (Crocodylus siamensis) . Protein J 31, 466–476 (2012). https://doi.org/10.1007/s10930-012-9424-7

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  • DOI: https://doi.org/10.1007/s10930-012-9424-7

Keywords

  • Antibacterial activity
  • Antioxidant activity
  • Crocodylus siamensis
  • Hemoglobin