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Effects of Furanocoumarins from Apiaceous Vegetables on the Catalytic Activity of Recombinant Human Cytochrome P-450 1A2

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Abstract

Inhibition of cytochrome P-450 1A2 (CYP1A2)-mediated activation of procarcinogens may be an important chemopreventive mechanism. Consumption of apiaceous vegetables (rich in furanocoumarins) inhibits CYP1A2 in humans. Because many furanocoumarins are potent inhibitors of several CYPs, we characterized the effects of three furanocoumarins from apiaceous vegetables on human CYP1A2 (hCYP1A2). We assessed hCYP1A2 methoxyresorufin O-demethylase (MROD) activity using microsomes from Saccharomyces cerevisiae expressing hCYP1A2. Isopimpinellin exhibited mechanism-based inactivation (MBI) of hCYP1A2 (K i  = 1.2 μM, k inact = 0.34 min−1, and partition ratio = 8). Imperatorin and trioxsalen were characterized as mixed inhibitors with K i values of 0.007 and 0.10 μM, respectively. These results indicate that even if present at low levels in apiaceous vegetables, imperatorin, trioxsalen and isopimpinellin may contribute significantly to CYP1A2 inhibition and potentially decreased procarcinogen activation. Moreover, the in vivo effect of isopimpinellin on CYP1A2 may be longer lasting compared to reversible inhibitors.

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Abbreviations

5-MOP:

5-Methoxypsoralen

8-MOP:

8-Methoxypsoralen

CYP:

Cytochrome P-450

CYP1A2:

Cytochrome P-450 1A2

DMSO:

Dimethyl sulfoxide

HBSS:

Hank’s balanced salt solution

hCYP1A2:

Human cytochrome P-450 1A2

MBI:

Mechanism-based inactivator

MROD:

Methoxyresorufin O-demethylase

NADPH:

β-Nicotinamideadenine dinucleotidephosphate

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Acknowledgments

The authors gratefully acknowledge and thank Dr. Patrick Hanna (Department of Medicinal Chemistry, University of Minnesota) and Dr. Linda von Weymarn (Masonic Cancer Center, University of Minnesota) for their expert guidance. The authors also thank Li Liu for assistance with development of assays. This work has been supported in part by the Minnesota Agricultural Experiment Station and by Award Number T32CA132670 from the National Cancer Institute. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institutes of Health.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, University of Minnesota, 225 Food Science and Nutrition, 1334 Eckles Avenue, St. Paul, MN, 55108-1038, USA

    Ah-Young Kang, Lindsay R. Young, Carlus Dingfelder & Sabrina Peterson

  2. Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA

    Sabrina Peterson

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  1. Ah-Young Kang
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  2. Lindsay R. Young
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  4. Sabrina Peterson
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Correspondence to Sabrina Peterson.

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Kang, AY., Young, L.R., Dingfelder, C. et al. Effects of Furanocoumarins from Apiaceous Vegetables on the Catalytic Activity of Recombinant Human Cytochrome P-450 1A2. Protein J 30, 447–456 (2011). https://doi.org/10.1007/s10930-011-9350-0

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