Skip to main content

Advertisement

SpringerLink
Log in

Proteomic Analysis of Differentially Expressed Proteins between Stenotic and Normal Colon Segment Tissues Derived from Patients with Hirschsprung’s Disease

  • Published:
The Protein Journal Aims and scope Submit manuscript

Abstract

Hirschsprung’s disease (HSCR) is the most common identifiable developmental disorder of the enteric nervous system. The present study was designed to analyze the differential proteomic patterns in stenotic colon segment tissues from patients with HSCR. We analyzed 20 paired stenotic and normal colon segment tissues from patients with HSCR, and identified 13 proteins from stenotic segment tissues peptide fingerprint mapping and SELDI MS that were separated using 2-DE. The protein levels of four selected proteins (α-actinin-4, ACTN4; myosin regulatory light chain interacting protein, MYLIP; fatty acid binding protein 7, FABP7; bone morphogenetic protein receptor type 1A, BMPR1A) were further validated by Western blot analysis. This study, investigating for the first time proteomic changes in stenotic colon segment tissues from patients with HSCR, provides potential markers or promising new candidate actors for the pathogenesis of HSCR.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

HSCR:

Hirschsprung’s disease

2-DE:

Two-dimensional electrophoresis

MALDI-TOF-MS:

Matrix assisted laser desorption ionisation time-of-flight mass spectrometry

References

  1. Amiel J, Lyonnet S (2001) J Med Genet 38:729–739

    Article  CAS  Google Scholar 

  2. Brewer KC, Mwizerva O, Goldstein AM (2005) Hum Pathol 36:1120–1126

    Article  CAS  Google Scholar 

  3. Chatterji B, Borlak J (2009) Proteomics 9:1044–1056

    Article  CAS  Google Scholar 

  4. Dai S, Wang Z, Pan X, Chen X, Wang W, Ren H, Feng Q, He JC, Han B, Chen N (2009) Nephron Clin Pract 111:c87–c94

    Article  CAS  Google Scholar 

  5. Dai S, Wang Z, Pan X, Wang W, Chen X, Ren H, Hao C, Han B, Chen N (2010) Nephrol Dial Transplant 25:824–835

    Article  CAS  Google Scholar 

  6. Gao H, He R, He X, Zhang Z, Wang D, Lv L, Wang W, Huang Y (2011) Int J Colorectal Dis 26:117–125

    Article  Google Scholar 

  7. Garcia-Barcelo MM, Tang CS, Ngan ES, Lui VC, Chen Y, So MT, Leon TY, Miao XP, Shum CK, Liu FQ, Yeung MY, Yuan ZW, Guo WH, Liu L, Sun XB, Huang LM, Tou JF, Song YQ, Chan D, Cheung KM, Wong KK, Cherny SS, Sham PC, Tam PK (2009) Proc Natl Acad Sci USA 106:2694–2699

    Article  CAS  Google Scholar 

  8. Haricharan RN, Georgeson KE (2008) Semin Pediatr Surg 17:266–275

    Article  Google Scholar 

  9. Henderson JM, Alexander MP, Pollak MR (2009) J Am Soc Nephrol 20:961–968

    Article  CAS  Google Scholar 

  10. Honda K, Yamada T, Endo R, Ino Y, Gotoh M, Tsuda H, Yamada Y, Chiba H, Hirohashi S (1998) J Cell Biol 140:1383–1393

    Article  CAS  Google Scholar 

  11. Kapur RP (2009) Semin Pediatr Surg 18:212–223

    Article  Google Scholar 

  12. Khotin M, Turoverova L, Aksenova V, Barlev N, Borutinskaite VV, Vener A, Bajenova O, Magnusson KE, Pinaev GP, Tentler D (2010) Biochem Biophys Res Commun 397:192–196

    Article  CAS  Google Scholar 

  13. Lee NP, Chen L, Lin MC, Tsang FH, Yeung C, Poon RT, Peng J, Leng X, Beretta L, Sun S, Day PJ, Luk JM (2009) J Proteome Res 8:1293–1303

    Article  CAS  Google Scholar 

  14. Lyonnet S, Bolino A, Pelet A, Abel L, Nihoul-Fekete C, Briard ML, Mok-Siu V, Kaariainen H, Martucciello G, Lerone M et al (1993) Nat Genet 4:346–350

    Article  CAS  Google Scholar 

  15. Martucciello G (2008) Eur J Pediatr Surg 18:140–149

    Article  CAS  Google Scholar 

  16. Olsson PA, Bornhauser BC, Korhonen L, Lindholm D (2000) Biochem Biophys Res Commun 279:879–883

    Article  CAS  Google Scholar 

  17. Olsson PA, Korhonen L, Mercer EA, Lindholm D (1999) J Biol Chem 274:36288–36292

    Article  CAS  Google Scholar 

  18. Schuchardt A, D’Agati V, Larsson-Blomberg L, Costantini F, Pachnis V (1995) J Intern Med 238:327–332

    Article  CAS  Google Scholar 

  19. Welsch T, Keleg S, Bergmann F, Bauer S, Hinz U, Schmidt J (2009) Pancreas 38:968–976

    Article  CAS  Google Scholar 

  20. Yamamoto S, Tsuda H, Honda K, Onozato K, Takano M, Tamai S, Imoto I, Inazawa J, Yamada T, Matsubara O (2009) Mod Pathol 22:499–507

    Article  CAS  Google Scholar 

  21. Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, Fu G, Shen Y, Fan HY, Lu G, Zhong M, Xu XR, Han ZG, Zhang JW, Tao J, Huang QH, Zhou J, Hu GX, Gu J, Chen SJ, Chen Z (2000) Genome Res 10:1546–1560

    Article  CAS  Google Scholar 

  22. Zhao Y, Ben H, Qu S, Zhou X, Yan L, Xu B, Zhou S, Lou Q, Ye R, Zhou T, Yang P, Qu D (2010) Proteome Sci 8:28

    Article  Google Scholar 

  23. Zhu X, Ding M, Yu ML, Feng MX, Tan LJ, Zhao FK (2010) BMC Cancer 10:290

    Article  Google Scholar 

  24. Zinkin NT, Grall F, Bhaskar K, Otu HH, Spentzos D, Kalmowitz B, Wells M, Guerrero M, Asara JM, Libermann TA, Afdhal NH (2008) Clin Cancer Res 14:470–477

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grant from the National Natural Science Foundation of China (No. 30772277). The authors are grateful to Prof. Weilin Wang and Prof. Lianying Wang of the Department of Pediatric Surgery, Shengjing Hospital Affiliated to China Medical University for their expert technical assistance.

Author information

Authors and Affiliations

  1. Key Laboratory of Health Ministry for Congenital Malformations, Shengjing Hospital Affiliated to China Medical University, 36 Sanhao Street, 110004, Shenyang, Liaoning Province, China

    Hong Gao & Mei Wu

  2. Department of Pharmacology, Shengjing Hospital Affiliated to China Medical University, 110004, Shenyang, Liaoning Province, China

    Xiaojing He

  3. Department of Pediatric Surgery, Shengjing Hospital Affiliated to China Medical University, 110004, Shenyang, Liaoning Province, China

    Zhibo Zhang, Dajia Wang, Liangying Lv & Ying Huang

  4. Department of Pediatric Surgery, Women and Children’s Hospital, 118002, Dandong, Liaoning Province, China

    Zhenwei Su

Authors
  1. Hong Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaojing He
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mei Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhibo Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dajia Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Liangying Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhenwei Su
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ying Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hong Gao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gao, H., He, X., Wu, M. et al. Proteomic Analysis of Differentially Expressed Proteins between Stenotic and Normal Colon Segment Tissues Derived from Patients with Hirschsprung’s Disease. Protein J 30, 138–142 (2011). https://doi.org/10.1007/s10930-011-9314-4

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10930-011-9314-4

Keywords

Search

Navigation