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Purification and Characterization of a Recombinant Bacteriophytochrome of Xanthomonas oryzae pathovar oryzae

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Abstract

Phytochrome-like proteins have been recently identified in prokaryotes but their features and functions are not clear. We cloned a gene encoding the phytochrome-like protein (XoBphP) in a pathogenic bacteria, Xanthomonas oryzae pv. oryzae (Xoo) and investigated characteristics of the protein using a recombinant XoBphP. The N-terminal region of XoBphP containing the PAS/GAF/PHY domains is highly similar to most bacteriophytochromes, but Cys4, corresponding to Cys24 of DrBphP, isn’t involved in chromophore attachment. Recombinant XoBphP could bind a bilin molecule and a differential spectrum from Pr/Pfr shows that XoBphP has similar characteristics of known bacteriophytochromes with shifted absorption maxima of 683 and 757 nm for the Pr and Pfr forms. Unlike other bacteriophytochromes, XoBphP has no histidine kinase domain at C-terminus. The domain was predicted from amino-acid 279 to 342 with less significance than the required threshold. This result suggests that XoBphP probably has different signal transduction mechanisms for its intracellular function.

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Abbreviations

Xoo :

Xanthomonas oryzae pv. oryzae

XoBphP:

Bacteriophytochrome of Xanthomonas oryzae pv. oryzae

XoBphP :

Bacteriophytochrome gene (PXO_03310) of Xanthomonas oryzae pv. oryzae

CBD:

Chromophore binding domain

Cph1:

Cyanobacterialphytochrome

CikA:

Circadian input kinase

GAF:

cGMP-specific phosphodiesterases, Adenylyl cyclases, and E.coli FhlA

TCS:

Two-component regulatory system

HK:

Histidine kinase

RR:

Rsponsive regulator

NMR:

Nuclear magnetic resonance

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Acknowledgment

This work was supported by a grant (20100613) from Kyung Hee University, Yongin, Korea.

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Authors and Affiliations

  1. Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Korea

    Man-Ho Cho & Seong Hee Bhoo

  2. Department of Plant Molecular Systems Biotech & Crop Biotech Institute, Kyung Hee University, Yongin, 446-701, Korea

    Youngchul Yoo & Sang-Won Lee

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  1. Man-Ho Cho
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  2. Youngchul Yoo
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  3. Seong Hee Bhoo
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  4. Sang-Won Lee
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Correspondence to Sang-Won Lee.

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Cho, MH., Yoo, Y., Bhoo, S.H. et al. Purification and Characterization of a Recombinant Bacteriophytochrome of Xanthomonas oryzae pathovar oryzae . Protein J 30, 124–131 (2011). https://doi.org/10.1007/s10930-011-9312-6

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