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Molecular Cloning and Expression of Aven Gene in Chicken

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Abstract

Aven was originally identified as a protein that regulates apoptosis by binding to apoptotic regulators, Bcl-xL and Apaf-1. Recently was found that Aven protein is a potent activator of ATM, critical for its DNA damage-induced activation. An Aven cDNA clone was isolated from chicken (Gallus gallus) after screening of a cerebellum cDNA library. The full-length cDNA is 1,430 nt in size, encoding for a polypeptide of 352 amino acid residues. The predicted amino acid sequence of the chicken Aven is 69, 46, 45 and 37% identical to those of zebra finch, human, xenopus and zebrafish orthologs, respectively. Expression analysis reveals that the chicken Aven gene is expressed in the adult brain, heart, intestine, kidney, lung, stomach and spleen, as well as in the whole embryos of 4- and 6-days old. Phylogenetic analysis of the Aven ortholog proteins from various organisms clusters the chicken Aven in the same group with other bird Avens.

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Abbreviations

Bcl-xL:

B-cell lymphoma-X long

Apaf-1:

Apoptotic protease-activating factor-1

ATM:

Ataxia-telangiectasia-mutated

NJ:

Neighbor-joining

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

This work is supported by University of Patras. The sequence described in this paper has been deposited in the EMBL database, GenBank accession no. AJ583692 (chicken Aven-like cDNA).

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Authors and Affiliations

  1. Department of Biology, School of Medicine, University of Patras, Rion, 26 500, Patras, Greece

    Elena Vezyri, Angeliki Mikrou, Aglaia Athanassiadou & Ioannis K. Zarkadis

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  1. Elena Vezyri
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  2. Angeliki Mikrou
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  3. Aglaia Athanassiadou
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  4. Ioannis K. Zarkadis
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Correspondence to Ioannis K. Zarkadis.

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Vezyri, E., Mikrou, A., Athanassiadou, A. et al. Molecular Cloning and Expression of Aven Gene in Chicken. Protein J 30, 72–76 (2011). https://doi.org/10.1007/s10930-011-9304-6

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