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Effects of Low Molecular Weight Sulfated Galactan Fragments From Botryocladia Occidentalis on the Pharmacological and Enzymatic Activity of Spla2 From Crotalus Durissus Cascavella

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Abstract

Low molecular weight fragments of sulfated galactans (Boc-5 and Boc-10) from the red algae Botryocladia occidentalis significantly inhibited Crotalus durissus cascavella sPLA2 enzymatic activity. Equimolar ratios of sPLA2 to Boc-5 or Boc-10 resulted in allosteric inhibition of sPLA2. Under the conditions tested, we observed that both Boc-5 and Boc-10 strongly decreased edema, myonecrosis, and neurotoxicity induced by native sPLA2.

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Fig. 1
Fig. 2

Abbreviations

sPLA2:

Secretory phospholipasis A2

Boc:

Sulfated galactan fragment of Botryocladia occidentalis

B. occidentalis :

Botryocladia occidentalis

C. durissus cascavella :

Crotalus durissus cascavella

SDS–PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

Maldi-Tof:

Matrix-assisted laser desorption/ionization, time-of-flight mass spectrometer

CD:

Circular dichroism

HPLC:

High-performance liquid chromatography or high-pressure liquid chromatography

[14C] 5-HT:

[14C] 5-hydroxytryptamine

CK:

Creatine kinase

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Acknowledgments

FAPEPS 2007 54714-3, CNPq 558193/2009-9 for financial support.

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Authors and Affiliations

  1. UNESP, Campus do Litoral Paulista, São Vicente, Brasil

    M. H. Toyama, S. C. B. Oliveira, F. H. R. Fagundes & E. B. S. Diz Filho

  2. Universidade Presbiteriana Mackenzie, São Paulo, Brasil

    D. O. Toyama

  3. Departamento de Engenharia de Pesca and/BioMol/BioMar, UFC, Fortaleza, Ceará, Brasil

    V. M. Torres, G. C. Pontes & W. R. L. Farias

  4. Hospital Universitário Clementino Fraga Filho (HUCFF)/UFRJ, Rio de Janeiro, Brasil

    F. R. Melo

  5. Departamento de Bioquímica Inst. Biologia, Unicamp Campinas, São Paulo, Brasil

    S. C. B. Oliveira, F. H. R. Fagundes & E. B. S. Diz Filho

  6. Departamento de Bioquímica, UFC, São Paulo, Brasil

    B. S. Cavada

Authors
  1. M. H. Toyama
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  2. D. O. Toyama
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  3. V. M. Torres
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  4. G. C. Pontes
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  5. W. R. L. Farias
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  6. F. R. Melo
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  7. S. C. B. Oliveira
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  8. F. H. R. Fagundes
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  9. E. B. S. Diz Filho
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  10. B. S. Cavada
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Corresponding author

Correspondence to E. B. S. Diz Filho.

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Toyama, M.H., Toyama, D.O., Torres, V.M. et al. Effects of Low Molecular Weight Sulfated Galactan Fragments From Botryocladia Occidentalis on the Pharmacological and Enzymatic Activity of Spla2 From Crotalus Durissus Cascavella . Protein J 29, 567–571 (2010). https://doi.org/10.1007/s10930-010-9294-9

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  • DOI: https://doi.org/10.1007/s10930-010-9294-9

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